Document Detail


Nuclear transplantation of ectodermal cells in pig oocytes: ultrastructure and radiography.
MedLine Citation:
PMID:  8844696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigated the changes in nucleolar fine structure and the synthesis of both heterogeneous nuclear RNA (hnRNA) and ribosomal RNA (rRNA) in pig reconstructed embryos obtained by electrofusion of a single pig ectodermal cell to an enucleated metaphase-II oocyte. The nucleolar morphological changes and the pattern of transcription were examined in the ectodermal cells before fusion and in the nuclear transfer reconstructed embryos 16-18 hr after fusion. Before fusion the ectodermal cells exhibited a reticulated nucleolus with active RNA synthesis. In the reconstructed embryos, modifications of the nucleolar structure were observed, as assessed by the presence of either round-shaped, compact, dense nucleolar precursor bodies, or reticulated nucleoli. However, in both cases there was no RNA synthesis. Blebbing activity of the nuclear envelope was not observed. These results indicate that the nucleus of pig ectodermal cells exhibited either a complete or an incomplete remodelling when transferred to an enucleated metaphase-II oocyte, with no detectable RNA synthesis. Cell-cycle synchronization of ectodermal cell donor nuclei may play an important role in nuclear reprogramming after fusion.
Authors:
N Ouhibi; J Fulka; J Kanka; R M Moor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  44     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-01-02     Completed Date:  1997-01-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  533-9     Citation Subset:  IM    
Affiliation:
Development and Differentiation Loboratory, Babraham Institute, Cambridge, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleolus / ultrastructure
Cell Nucleus / metabolism
Cells, Cultured
Chromatin / ultrastructure
Ectoderm / transplantation*,  ultrastructure
Nuclear Transfer Techniques*
Oocytes / radiography,  ultrastructure*
RNA / biosynthesis
Swine
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Chromatin; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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