Document Detail


Nuclear translocation of immulectin-3 stimulates hemocyte proliferation.
MedLine Citation:
PMID:  18282603     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immulectin-3 (IML-3) is a C-type lectin from the tobacco hornworm Manduca sexta that contains a motif (NWGV) similar to the BH1 motif (NWGR) of the mammalian galectin-3. IML-3 is synthesized in fat body and secreted into hemolymph, but can be translocated into hemocytes. In this study, we showed that IML-3 was predominantly localized to the nucleus of hemocytes and some metaphase, anaphase and telophase hemocytes from M. sexta larvae injected with bacterial lipopolysaccharide (LPS). IML-3 was detected in the membrane and soluble extracts of hemocytes, suggesting that it may be translocated into hemocytes via receptor-mediated endocytosis. To investigate the role of IML-3 translocation to the nucleus, we expressed recombinant wild-type IML-3 and a deletion mutant DeltaIML-3 that has the NWGV motif deleted in Drosophila S2 cells. We found that recombinant wild-type IML-3, but not DeltaIML-3, was localized to the nucleus of some S2 cells and also detected in the nuclear extract. Expression of recombinant wild-type IML-3, but not DeltaIML-3 or GFP, increased the number of proliferating S2 cells. Our results suggest that nuclear translocation of IML-3 may stimulate hemocyte proliferation.
Authors:
Erjun Ling; Jingqun Ao; Xiao-Qiang Yu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-02-20
Journal Detail:
Title:  Molecular immunology     Volume:  45     ISSN:  0161-5890     ISO Abbreviation:  Mol. Immunol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-03-24     Completed Date:  2008-07-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2598-606     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA.
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MeSH Terms
Descriptor/Qualifier:
Active Transport, Cell Nucleus
Animals
Cell Nucleus / immunology,  metabolism*
Cell Proliferation
Endocytosis
Hemocytes / cytology*,  immunology*,  metabolism
Hemolymph / immunology,  metabolism
Insect Proteins / immunology,  metabolism*
Larva / cytology,  drug effects,  immunology,  metabolism*
Lectins, C-Type / immunology,  metabolism*
Lipopolysaccharides / immunology
Manduca / cytology,  immunology*,  metabolism
Mutant Proteins / immunology,  metabolism
Recombinant Proteins / immunology,  metabolism
Grant Support
ID/Acronym/Agency:
GM66356/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Insect Proteins; 0/Lectins, C-Type; 0/Lipopolysaccharides; 0/Mutant Proteins; 0/Recombinant Proteins; 0/immulectin, Manduca sexta

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