Document Detail

Nuclear translocation of Fos is stimulated by interaction with Jun through the leucine zipper.
MedLine Citation:
PMID:  10188588     Owner:  NLM     Status:  MEDLINE    
Jun and Fos, b-ZIP transcription factors, form a heterodimer and bind to DNA enhancer elements, thereby regulating the expression of target genes. The present study was undertaken to investigate the molecular mechanism underlying nuclear translocation of the Jun/Fos complex. For this purpose, normal rat kidney cells were microinjected with a DNA expression vector containing wild-type or mutant c- or v-jun together with c- or v-fos, followed by detection of the subcellular localization of Jun or Fos by immunofluorescence staining. The nuclear accumulation of Fos was markedly enhanced by the presence of wildtype Jun, but not by Jun mutants lacking nuclear targeting or zipper dimerization functions, implying that Jun and Fos mutually interact via their leucine zippers and translocate from the cytoplasm to the nucleus using the markedly stronger nuclear localization signal of Jun.
K Chida; S Nagamori; T Kuroki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  55     ISSN:  1420-682X     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-04-20     Completed Date:  1999-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  297-302     Citation Subset:  IM    
Department of Cancer Cell Research, University of Tokyo, Japan.
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MeSH Terms
Biological Transport
Cell Line
Cell Nucleus / metabolism
Gene Expression
Leucine Zippers*
Oncogene Protein p65(gag-jun) / genetics,  metabolism*
Oncogene Proteins v-fos / genetics,  metabolism*
Proto-Oncogene Proteins c-fos / genetics,  metabolism*
Proto-Oncogene Proteins c-jun / genetics,  metabolism*
Reg. No./Substance:
0/Oncogene Protein p65(gag-jun); 0/Oncogene Proteins v-fos; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun

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