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Nuclear transferred embryonic stem cell for analysis of B1 B-lymphocyte development.
MedLine Citation:
PMID:  23042789     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The transfer of nuclei of fully differentiated cells into enucleated oocytes is a well-recognized method for the generation of embryonic stem (ES) cells. Here, we demonstrate that nuclear transferred ES (NT-ES) cells can be established with high efficiency using innate-like B lymphocytes as donor cells. We established two mouse lines carrying rearranged Ig heavy and light chains using NT-ES cells containing nuclei from peritoneal cavity B1 cells. Analysis of B1 clone lines revealed that the B1-cell generation critically depends on the interaction between antigen (possibly self-antigen) and surface Ig, while the B1-cell maintenance requires the peritoneal environment. The B1-cell expansion takes place in spleen, and is held in check by competitor B2 cells. The results indicate that the NT-ES method could replace the transgenic or knock-in mouse approaches currently used to study the biology of cells that undergo somatic rearrangements of their antigen receptor genes.
Authors:
Mitsuyo Takase; Ryuji Iida; Mikako Maruya; Asako Sakaue-Sawano; Atsushi Miyawaki; Teruhiko Wakayama; Satoshi Nishigami; Sidonia Fagarasan; Osami Kanagawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-6
Journal Detail:
Title:  International immunology     Volume:  -     ISSN:  1460-2377     ISO Abbreviation:  Int. Immunol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8916182     Medline TA:  Int Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Laboratory for Autoimmune Regulation, RIKEN, Research Center for Allergy and Immunology, 1-7-22 Suehiro-cho, Yokohama, Kanagawa 230-0045, Japan.
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