Document Detail


Nuclear transfer and oocyte cryopreservation.
MedLine Citation:
PMID:  19152744     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Somatic cells can be reprogrammed to a totipotent state through nuclear transfer or cloning, because it has been demonstrated that the oocyte has the ability to reprogramme an adult nucleus into an embryonic state that can initiate the development of a new organism. Therapeutic cloning, whereby nuclear transfer is used to derive patient-specific embryonic stem cells, embraces an entire new opportunity for regenerative medicine. However, a key obstacle for human therapeutic cloning is that the source of fresh human oocytes is extremely limited. In the present review, we propose prospective sources of human oocytes by using oocyte cryopreservation, such as an oocyte bank and immature oocytes. We also address some potential issues associated with nuclear transfer when using cryopreserved oocytes. In the future, if the efficacy and efficiency of cryopreserved oocytes are comparable to those of fresh oocytes in human therapeutic cloning, the use of cryopreserved oocytes would be invaluable and generate a great impact to regenerative medicine.
Authors:
Ching-Chien Chang; Li-Ying Sung; Tomokazu Amano; X Cindy Tian; Xiangzhong Yang; Zsolt Peter Nagy
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  21     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  2009  
Date Detail:
Created Date:  2009-01-20     Completed Date:  2009-04-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  37-44     Citation Subset:  IM    
Affiliation:
Reproductive Biology Associates, 1150 Lake Hearn Dr, Suite 400, Atlanta, GA 30342, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cryopreservation / methods*
Humans
Nuclear Transfer Techniques*
Oocytes / cytology*
Regenerative Medicine / methods*,  trends

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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