Document Detail


Nuclear transfer methods to study aging.
MedLine Citation:
PMID:  17634583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maternal age affects oocyte quality and early embryo development. Aberrant meiosis of oocytes and compromised early embryo development from older females could originate from defects in the nucleus, the cytoplasm, or both. Nuclear transfer has been used for decades as a tool to study nuclear-cytoplasmic interactions in early embryos, and has uncovered genomic imprinting, nuclear reprogramming, and produced animal clones. Here, we describe the technique for investigating nuclear-cyoplasmic interactions in oocytes and zygotes in female reproductive aging. Nuclear transfer can be performed efficiently and effects of the technique itself on meiosis and early embryo development are minimal as long as care is taken to minimize insult to oocytes or embryos. This protocol first focuses on use of nuclear transfer to study nucleus versus cytoplasmic origin in agingassociated meiosis defects in oocytes at the germinal vesicle (GV) stage. Then, nuclear transfer is used at the zygote stage to study nuclear and cytoplasmic abnormality and apoptosis in early development.
Authors:
Lin Liu; David L Keefe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  371     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-19     Completed Date:  2007-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  191-207     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Women &Infants Hospital, Brown Medical School, Providence, RI, USA.
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MeSH Terms
Descriptor/Qualifier:
Aging / metabolism*,  pathology
Animals
Apoptosis
Cell Nucleus / metabolism*,  pathology
Cytoplasm / metabolism,  pathology
Female
Meiosis
Models, Biological*
Nuclear Transfer Techniques*
Oocytes / metabolism*,  pathology
Sheep
Zygote / metabolism*,  pathology

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