| Nuclear transfer methods to study aging. | |
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MedLine Citation:
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PMID: 17634583 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Maternal age affects oocyte quality and early embryo development. Aberrant meiosis of oocytes and compromised early embryo development from older females could originate from defects in the nucleus, the cytoplasm, or both. Nuclear transfer has been used for decades as a tool to study nuclear-cytoplasmic interactions in early embryos, and has uncovered genomic imprinting, nuclear reprogramming, and produced animal clones. Here, we describe the technique for investigating nuclear-cyoplasmic interactions in oocytes and zygotes in female reproductive aging. Nuclear transfer can be performed efficiently and effects of the technique itself on meiosis and early embryo development are minimal as long as care is taken to minimize insult to oocytes or embryos. This protocol first focuses on use of nuclear transfer to study nucleus versus cytoplasmic origin in agingassociated meiosis defects in oocytes at the germinal vesicle (GV) stage. Then, nuclear transfer is used at the zygote stage to study nuclear and cytoplasmic abnormality and apoptosis in early development. |
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Authors:
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Lin Liu; David L Keefe |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 371 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-07-19 Completed Date: 2007-09-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 191-207 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Women &Infants Hospital, Brown Medical School, Providence, RI, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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metabolism*,
pathology Animals Apoptosis Cell Nucleus / metabolism*, pathology Cytoplasm / metabolism, pathology Female Meiosis Models, Biological* Nuclear Transfer Techniques* Oocytes / metabolism*, pathology Sheep Zygote / metabolism*, pathology |
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