Document Detail

Nuclear texture and chromatin structure in OV1/VCR human multidrug-resistant cell line.
MedLine Citation:
PMID:  12964008     Owner:  NLM     Status:  MEDLINE    
Previous studies have demonstrated that multidrug-resistant leukemic cells displayed nuclear texture changes. In this work, the human ovarian carcinoma cell line IGROV1 and its multidrug-resistant variant OV1/VCR were studied. Cell smears of these cell populations were analysed by image cytometry. As compared to sensitive cells, OV1/VCR display a chromatin global decondensation as assessed by textural features analysis. In order to correlate this decondensation with alterations in chromatin structure, DNase I was used. OV1/VCR DNA displayed an increased DNase I sensitivity, suggesting an increased chromatin accessibility. Furthermore, OV1/VCR cells displayed an increased level in acetylated histone H4, a mechanism known to be associated with transcriptionally active chromatin and relaxed chromatin conformation.
Sonia Yatouji; Aurelie Trussardi-Regnier; Chantal Trentesaux; Françoise Liautaud-Roger; Jean Dufer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  23     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-09     Completed Date:  2004-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1225-30     Citation Subset:  IM    
Unité MéDIAN-CNRS UMR 6142, IFR 53, Faculté de Pharmacie, 51096 Reims cedex, France.
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MeSH Terms
Cell Line, Tumor
Cell Nucleus / ultrastructure*
Chromatin / chemistry*,  metabolism
Deoxyribonuclease I / metabolism
Drug Resistance, Multiple*
Drug Resistance, Neoplasm*
Histones / chemistry
Image Cytometry
Ovarian Neoplasms / pathology
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Chromatin; 0/Histones; EC I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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