Document Detail

Nuclear survivin is associated with cell proliferative advantage in uterine cervical carcinomas during radiation therapy.
MedLine Citation:
PMID:  22389513     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Although the anticancer effects of radiation therapy for patients with uterine cervical squamous cell carcinoma (U-SCC) are widely acknowledged, little is known about the resultant morphological alterations in tumour tissue kinetics.
AIMS: To make a detailed assessment of possible roles of survivin expression in apoptosis and cell proliferation in U-SCC during radiation therapy.
METHODS: 181 biopsy specimens from 55 consecutive U-SCCs of patients receiving radiation therapy were studied using a combined morphological (apoptosis) and immunohistochemical (MIB-1 and survivin) approach. The intracellular distribution of various splice variants of the survivin gene was also examined.
RESULTS: Tumour cell proliferation, determined as MIB-1 labelling indices (LIs), as well as nuclear survivin (N-Surv) LIs, were inversely correlated with irradiation dosage, in contrast to relatively minor changes in apoptotic indices, suggesting a shift in tumour tissue kinetics towards a relative predominance of cell deletion. In addition, the low N-Sur LI category showed significant stepwise decrease in MIB-1 LIs during therapy, in contrast to no changes in the high category. Exogenous overexpression of three variants of the survivin gene resulted in different expression patterns, showing cytoplasmic staining with or without dot formation for survivin and survivin-2B and distinct nuclear accumulation for survivin-deled exon 3 (Ex3).
CONCLUSIONS: Results showed that nuclear survivin, including survivin itself and the survivin-Ex3 splice variants, may participate in modulation of altered cell kinetics of U-SCC during radiation therapy.
Pattama Chaopotong; Sabine Kajita; Miki Hashimura; Makoto Saegusa
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Publication Detail:
Type:  Journal Article     Date:  2012-03-03
Journal Detail:
Title:  Journal of clinical pathology     Volume:  65     ISSN:  1472-4146     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-24     Completed Date:  2012-06-12     Revised Date:  2014-07-03    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  424-30     Citation Subset:  AIM; IM    
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MeSH Terms
Aged, 80 and over
Alternative Splicing / radiation effects
Apoptosis / radiation effects
Carcinoma, Squamous Cell / genetics,  pathology*,  radiotherapy
Cell Nucleus / metabolism,  pathology*
Cell Proliferation
DNA, Neoplasm / analysis
Gene Expression / radiation effects
Inhibitor of Apoptosis Proteins / genetics*
Middle Aged
Protein Isoforms / metabolism
Tumor Markers, Biological / genetics
Ubiquitin-Protein Ligases / genetics
Uterine Cervical Neoplasms / genetics,  pathology*,  radiotherapy
Reg. No./Substance:
0/BIRC5 protein, human; 0/DNA, Neoplasm; 0/Inhibitor of Apoptosis Proteins; 0/Protein Isoforms; 0/Tumor Markers, Biological; EC ligase, human; EC Ligases

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