Document Detail


Nuclear structure: determination of the fate of the nuclear envelope in Drosophila during mitosis using monoclonal antibodies.
MedLine Citation:
PMID:  6420424     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Libraries of monoclonal antibody against nuclear proteins of Drosophila melanogaster have been established recently to investigate nuclear structure and function. Some of the antibodies have been characterized as being directed against the nuclear envelope. Further studies detailed in this paper describe the fate of the nuclear envelope during mitosis. Indirect immunofluorescence staining of whole developing Drosophila embryos has been used as a system in which nuclear events can be studied both synchronously and in a longitudinal gradient of mitotic structures. The results show a pattern of breakdown and reconstruction of the nuclear envelope in which the antigen is always present in particulate structures. In addition, the processes of antigen rearrangement are shown to be spatially determined throughout mitosis.
Authors:
J P Fuchs; H Giloh; C H Kuo; H Saumweber; J Sedat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  64     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-03-23     Completed Date:  1984-03-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  331-49     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology
Antibody Specificity
Drosophila melanogaster / ultrastructure*
Fluorescent Antibody Technique
Interphase
Metaphase
Microscopy, Fluorescence
Mitosis*
Nuclear Envelope / immunology,  physiology*,  ultrastructure
Prophase
Telophase
Grant Support
ID/Acronym/Agency:
GM-25101/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal

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