Document Detail

Nuclear retinoic-acid-binding proteins and receptors in retinoic-acid-responsive cell lines.
MedLine Citation:
PMID:  2562438     Owner:  NLM     Status:  MEDLINE    
Nuclear retinoic-acid-binding activity and the expression of retinoic acid receptor mRNA (RAR-alpha and RAR-beta) were assayed in the F9 embryonal carcinoma, HeLa, HL-60 promyelocytic leukaemia and S91 melanoma cell lines. A 4-svedberg nuclear retinoic-acid-binding activity was detected in all 4 cell lines, but the levels in the HeLa and HL-60 cells were lower than in the F9 and S91 lines. RAR-alpha mRNA was expressed in all 4 cell lines, although at a very low level in S91 cells. Conversely, RAR-beta mRNA was expressed in S91 cells and, at a lower level, in F9 cells but was undetectable in HeLa and HL-60 cells. RAR-beta, transcribed and translated in vitro from the cloned cDNA coding region, sedimented at 4 S and this suggests that the 4-svedberg nuclear retinoic-acid-binding activity may represent the retinoic acid receptors.
A K Daly; J L Rees; C P Redfern
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell biology     Volume:  57     ISSN:  0304-3568     ISO Abbreviation:  Exp. Cell Biol.     Publication Date:  1989  
Date Detail:
Created Date:  1992-03-30     Completed Date:  1992-03-30     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  7701827     Medline TA:  Exp Cell Biol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  339-45     Citation Subset:  IM    
Department of Dermatology, University of Newcastle upon Tyne, Medical School, UK.
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MeSH Terms
Blotting, Northern
Carrier Proteins / analysis*
Cell Line / metabolism
Cell Nucleus / metabolism*
DNA Probes
Gene Expression
RNA, Messenger / analysis
Receptors, Retinoic Acid
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Carrier Proteins; 0/DNA Probes; 0/RNA, Messenger; 0/Receptors, Retinoic Acid

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