| Nuclear retention of fission yeast dicer is a prerequisite for RNAi-mediated heterochromatin assembly. | |
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MedLine Citation:
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PMID: 20152181 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RNaseIII ribonucleases act at the heart of RNA silencing pathways by processing precursor RNAs into mature microRNAs and siRNAs. In the fission yeast Schizosaccharomyces pombe, siRNAs are generated by the RNaseIII enzyme Dcr1 and are required for heterochromatin formation at centromeres. In this study, we have analyzed the subcellular localization of Dcr1 and found that it accumulates in the nucleus and is enriched at the nuclear periphery. Nuclear accumulation of Dcr1 depends on a short motif that impedes nuclear export promoted by the double-stranded RNA binding domain of Dcr1. Absence of this motif renders Dcr1 mainly cytoplasmic and is accompanied by remarkable changes in gene expression and failure to assemble heterochromatin. Our findings suggest that Dicer proteins are shuttling proteins and that the steady-state subcellular levels can be shifted toward either compartment. |
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Authors:
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Stephan Emmerth; Heiko Schober; Dimos Gaidatzis; Tim Roloff; Kirsten Jacobeit; Marc B?hler |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental cell Volume: 18 ISSN: 1878-1551 ISO Abbreviation: Dev. Cell Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-02-15 Completed Date: 2010-03-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101120028 Medline TA: Dev Cell Country: United States |
Other Details:
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Languages: eng Pagination: 102-13 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GEO/GSE18440; GSE18582 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Active Transport, Cell Nucleus
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genetics Amino Acid Motifs / genetics Binding Sites / genetics Cell Compartmentation / genetics Cell Nucleus / enzymology, genetics* Centromere / genetics, metabolism, ultrastructure Cytoplasm / enzymology, genetics Gene Expression Regulation, Fungal / genetics Heterochromatin / genetics* Protein Transport / genetics RNA Interference / physiology* RNA, Small Interfering / biosynthesis, genetics Ribonuclease III / genetics, metabolism* Schizosaccharomyces / enzymology, genetics* |
| Chemical | |
Reg. No./Substance:
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0/Heterochromatin; 0/RNA, Small Interfering; EC 3.1.26.3/Ribonuclease III |
| Comments/Corrections | |
Comment In:
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Dev Cell. 2010 Jan 19;18(1):6-7
[PMID:
20152173
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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