| Nuclear reprogramming in heterokaryons is rapid, extensive, and bidirectional. | |
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MedLine Citation:
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PMID: 19141533 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An understanding of nuclear reprogramming is fundamental to the use of cells in regenerative medicine. Due to technological obstacles, the time course and extent of reprogramming of cells following fusion has not been assessed to date. Here, we show that hundreds of genes are activated or repressed within hours of fusion of human keratinocytes and mouse muscle cells in heterokaryons, and extensive changes are observed within 4 days. This study was made possible by the development of a broadly applicable approach, species-specific transcriptome amplification (SSTA), which enables global resolution of transcripts derived from the nuclei of two species, even when the proportions of species-specific transcripts are highly skewed. Remarkably, either phenotype can be dominant; an excess of primary keratinocytes leads to activation of the keratinocyte program in muscle cells and the converse is true when muscle cells are in excess. We conclude that nuclear reprogramming in heterokaryons is rapid, extensive, bidirectional, and dictated by the balance of regulators contributed by the cell types. |
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Authors:
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Adam Palermo; Regis Doyonnas; Nidhi Bhutani; Jason Pomerantz; Ozan Alkan; Helen M Blau |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-01-13 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 23 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-01 Completed Date: 2009-05-26 Revised Date: 2013-03-18 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 1431-40 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology and Stem Cell Institute, Stanford University School of Medicine, 269 Campus Dr., Stanford, CA 94305-5175, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cell Fusion Gene Expression Profiling / methods* Humans Hybrid Cells / metabolism, physiology* Keratinocytes / physiology Mice Nuclear Reprogramming* Oligonucleotide Array Sequence Analysis |
| Grant Support | |
ID/Acronym/Agency:
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AF051678/AF/ACF HHS; AG009521/AG/NIA NIH HHS; AG020961/AG/NIA NIH HHS; AG024987/AG/NIA NIH HHS; HD018179/HD/NICHD NIH HHS; R01 AG009521/AG/NIA NIH HHS; R01 AG009521-25/AG/NIA NIH HHS |
| Comments/Corrections | |
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