Document Detail


Nuclear recruitment of A1p145 subunit of replication factor C in the early G1 phase of the cell cycle in Faza 567 hepatoma cell line and hepatocyte primary cultures.
MedLine Citation:
PMID:  7729533     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using a combination of immunoprecipitation and Western blotting with Faza 567 hepatoma cell extracts revealed that the large subunit of replication factor C (A1p145; mRFC140) was in a complex with proliferating cell nuclear antigen (PCNA). Western blotting showed that A1p145 was more abundant in nuclear extracts from butyrate-treated hepatoma cells which blocks the cells in the G1 phase of the cell cycle than from routinely cultured cells. Indirect immunoperoxidase analysis of G1 blocked Faza hepatoma cells localized A1p145 protein predominantly in the nucleoli. When hepatoma cells were stimulated to progress toward the S phase, A1p145 protein was then observed in both the cytoplasm and the nucleoplasm of these cells. Studies with early cultured normal hepatocytes which are progressing from G0 towards G1, also showed a nucleolus distribution for A1p145. This is the first demonstration in mammalian cells that the large subunit of replication factor C is associated with PCNA in the nucleus and that its distribution within cells changes during the cell cycle.
Authors:
F Levavasseur; P D Burbelo; S Cariou; J Liétard; Y Yamada; B Clément
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  363     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-05-30     Completed Date:  1995-05-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  132-6     Citation Subset:  IM    
Affiliation:
Unité de Recherches Hépatologiques U 49 de l'INSERM, CHRU Pontchaillou, Rennes, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Butyric Acid
Butyric Acids / pharmacology
Cell Nucleus / metabolism*
Cytoplasm / metabolism
DNA-Binding Proteins / analysis,  chemistry,  metabolism*
G1 Phase* / drug effects
Homeodomain Proteins*
Immunoenzyme Techniques
Immunosorbent Techniques
Liver / metabolism*,  ultrastructure
Liver Neoplasms, Experimental / metabolism*,  ultrastructure
Macromolecular Substances
Male
Proliferating Cell Nuclear Antigen / metabolism
Proto-Oncogene Proteins c-bcl-2*
Rats
Rats, Sprague-Dawley
Replication Protein C
Repressor Proteins*
Saccharomyces cerevisiae Proteins*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/BCL2-related protein A1; 0/Butyric Acids; 0/DNA-Binding Proteins; 0/Homeodomain Proteins; 0/MATA1 protein, S cerevisiae; 0/Macromolecular Substances; 0/Proliferating Cell Nuclear Antigen; 0/Proto-Oncogene Proteins c-bcl-2; 0/Replication Protein C; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins; 107-92-6/Butyric Acid

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