| Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat. A freeze-fracture study. | |
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MedLine Citation:
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PMID: 20925197 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe 'en face' the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still showing an even distribution on the nuclear membrane, never forming aggregations sharply separated from pore-free areas, which are characteristic of other apoptotic models. Electron microscopy of thin-sections shows, coincidently with findings in the freeze-fracture replicas, a moderately irregular aggregation of marginal heterochromatin condensations. After nuclear fragmentation and micronuclear formation, pores behave in the usual manner in other apoptotic models, i.e., mainly showing migrations of nuclear pores toward the chromatin-free areas. The present results support the hypothesis that nuclear pore complexes are dynamic structures, which permit their migration toward nuclear membrane areas devoid of chromatin aggregations that might block the nucleocytoplasmic transport in such areas. |
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Authors:
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Juan Carlos Cavicchia; Gustavo Guembe; Mabel Fóscolo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al Volume: 34 ISSN: 0327-9545 ISO Abbreviation: Biocell Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-10-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9438655 Medline TA: Biocell Country: Argentina |
Other Details:
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Languages: eng Pagination: 81-9 Citation Subset: IM |
Affiliation:
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Instituto de Histología y Embriología "Dr. Mario H. Burgos" (IHEM), CONICET, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Argentina. jccavic@fcm.uncu.edu.ar |
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