Document Detail


Nuclear morphometry in canine acanthomatous ameloblastomas and squamous cell carcinomas.
MedLine Citation:
PMID:  16864123     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to evaluate whether morphometrical analysis can be of diagnostic value for canine acanthomatous ameloblastoma. We calculated, by means of an automated image analyser, some morphometric nuclear parameters, in particular: mean nuclear area (MNA), mean nuclear perimeter (MNP), maximum and minimum diameters (MDx and MDm) coefficient of variation of the nuclear area (NACV), largest to smallest dimension ratio (LS ratio), and form factor (FF), in 8 canine acanthomatous ameloblastomas, and we compared these morphometric data to those of 13 squamous cell carcinomas of canine gingiva. The results indicated a progressive increase of the MNA, NACV, MNP and MDm proceeding from acanthomatous ameloblastomas (MNA: 42.11+/-8.74; NACV: 28,36+/-7,23; MNP: 24.18+/- 2.68; MDm: 5.69+/-0.49) to squamous cell carcinomas (MNA:49,69+/-9,10; NACV: 30,89+/-7,75; MNP: 25.63+/-2.54; MDm: 6.64+/-0.73). On the contrary, the LS ratio and the FF resulted greater in acanthomatous ameloblastomas (LS ratio: 1,63+/-0,12; FF: 1,13+/-0,002) than in SCCs (LS ratio: 1,40+/-0,12; FF:0.91+/-0.38). Moreover, the MNA, MNP,MDx and MDm resulted similar (MNA: p=0.89; MNP: p=0,65; MDm: p=0,16; MDx: p=0,13) in a subset of four acanthomatous ameloblastomas with cellular atypia (MNA:49,01+/-6,88; MNP: 26,28+/-1,99; MDm: 6.08+/-0.41; MDx: 10.18+/-0.88) and in squamous cell carcinomas (MNA:49.69+/-9,10; MNP: 25.63+/-2.54; MDm: 6.64+/-0.73; MDx: 9.26+/-1.05). While the NACV values resulted higher in typical acanthomatous ameloblastoma (29,99+/-6,06) than in atypical acanthomatous ameloblastoma (26,74+/-8,84) and similar to those of the SCCs (30,89+/-7,75). These results seem to confirm that acanthomatous ameloblastoma is a malignant or potentially malignant lesion and emphasizes that nuclear morphometry analysis can be an useful diagnostic and prognostic method in canine oral pathology.
Authors:
M Martano; S Damiano; B Restucci; O Paciello; V Russo; P Maiolino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of histochemistry : EJH     Volume:  50     ISSN:  1121-760X     ISO Abbreviation:  Eur J Histochem     Publication Date:    2006 Apr-Jun
Date Detail:
Created Date:  2006-07-25     Completed Date:  2007-01-12     Revised Date:  2008-08-15    
Medline Journal Info:
Nlm Unique ID:  9207930     Medline TA:  Eur J Histochem     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  125-30     Citation Subset:  IM    
Affiliation:
Dipartimento di Patologia e Sanità Animale, Facoltà di Scienze Biotecnologiche, Università degli Studi di Napoli Federico II, Naples, Italy. manuela.martano@unina.it
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MeSH Terms
Descriptor/Qualifier:
Ameloblastoma / diagnosis,  pathology,  veterinary*
Animals
Carcinoma, Squamous Cell / diagnosis,  pathology,  veterinary*
Cell Nucleus / pathology*,  ultrastructure
Dog Diseases / diagnosis*,  pathology
Dogs
Gingiva / pathology*,  ultrastructure
Image Cytometry / methods
Jaw Neoplasms / diagnosis,  pathology,  veterinary*

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