| Nuclear morphology measurements with angle-resolved low coherence interferometry for application to cell biology and early cancer detection. | |
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MedLine Citation:
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PMID: 21988885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The study of intact, living cells using non-invasive optical spectroscopic methods offers the opportunity to assess cellular structure and organization in a way that is not possible with commonly used cell biology imaging techniques. We have developed a novel spectroscopic technique for diagnosing disease at the cellular level based on using low-coherence interferometry (LCI) to detect the angular distribution of scattered light. Angle-resolved LCI (a/LCI) combines the ability of LCI to isolate scattering from sub-surface tissue layers with the ability of light scattering spectroscopy to obtain structural information on sub-wavelength scales. In application to examining cellular structure, a/LCI enables quantitative measurements of changes in the size and texture of cell nuclei. These quantitative measurements are characteristic of different pathological states. The capabilities of a/LCI were demonstrated using a clinical system that can be applied in endoscopic surveillance of esophageal tissue, producing high sensitivity and specificity for detecting dysplastic tissues in vivo. Experiments with in vitro cell samples also show the utility of a/LCI in observing structural changes due to environmental stimuli as well as detecting apoptosis due to chemotherapeutic agents. |
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Authors:
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Adam Wax; Kevin J Chalut |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review |
Journal Detail:
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Title: Analytical cellular pathology (Amsterdam) Volume: 34 ISSN: 2210-7185 ISO Abbreviation: Anal Cell Pathol (Amst) Publication Date: 2011 |
Date Detail:
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Created Date: 2011-10-12 Completed Date: 2012-01-26 Revised Date: 2013-05-23 |
Medline Journal Info:
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Nlm Unique ID: 101541993 Medline TA: Anal Cell Pathol (Amst) Country: Netherlands |
Other Details:
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Languages: eng Pagination: 207-22 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708, USA. a.wax@duke.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / pharmacology Apoptosis / drug effects Barrett Esophagus / diagnosis*, pathology Cell Line, Tumor Cell Nucleus Shape* / drug effects Early Detection of Cancer Esophageal Neoplasms / diagnosis*, pathology Esophagoscopy* High-Throughput Screening Assays / methods* Humans Interferometry / methods* Light Precancerous Conditions / diagnosis*, pathology Predictive Value of Tests Scattering, Radiation Sensitivity and Specificity |
| Grant Support | |
ID/Acronym/Agency:
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R21 CA120128/CA/NCI NIH HHS; R21CA120128-01/CA/NCI NIH HHS; R33 CA109907/CA/NCI NIH HHS; R33-CA109907/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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