| Nuclear matrix composition and in vitro cellular senescence. | |
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MedLine Citation:
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PMID: 8026567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The synthesis of nuclear matrix components from human diploid fibroblasts of different in vitro ages was analyzed. Radiolabeled nuclear matrices were prepared from human diploid fibroblasts at various stages of the cell cycle, and their components were separated by two dimensional electrophoresis. The same general electrophoretic pattern was observed at all cell cycle points analyzed, regardless of in vitro age. However, several of the more than 150 peptides that were observed exhibited some cell cycle or age-related variation in radiolabeling. Ten of these were chosen for further analysis. One peptide, with an approximate molecular weight of 47 kDa and pI of 6.8 exhibited the most significant cell cycle and age-related alterations. In matrices from younger cells, incorporation into this peptide was very low in GO but increased as these cells moved through the cell cycle, with maximum incorporation occurring in S phase. As cells neared the end of their in vitro lifespan, labeling of this peptide was elevated at all stages of the cell cycle. Since many of the functional alterations observed in senescent human diploid fibroblasts are nuclear-matrix-associated activities, these results suggest that the inappropriate expression of nuclear matrix components contribute to the functional changes which characterize in vitro senescence. |
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Authors:
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R T Dell'Orco; W L Whittle |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental gerontology Volume: 29 ISSN: 0531-5565 ISO Abbreviation: Exp. Gerontol. Publication Date: 1994 Mar-Apr |
Date Detail:
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Created Date: 1994-08-05 Completed Date: 1994-08-05 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 139-49 Citation Subset: IM |
Affiliation:
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Oklahoma Medical Research Foundation, Oklahoma City 73104. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Nuclear Cell Aging / physiology* Cell Cycle Cell Line Fibroblasts / cytology, metabolism Humans Intermediate Filaments / chemistry, metabolism Molecular Weight Nuclear Matrix / chemistry, metabolism* Nuclear Proteins / chemistry, metabolism Peptides / chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Nuclear; 0/Nuclear Proteins; 0/Peptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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