Document Detail


Nuclear marginalization of host cell chromatin associated with expansion of two discrete virus-induced subnuclear compartments during baculovirus infection.
MedLine Citation:
PMID:  18434402     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromatin structure is strictly regulated during the cell cycle. DNA viruses occasionally disturb the spatial organization of the host cell chromatin due to formation of the viral DNA replication compartment. To examine chromatin behavior in baculovirus-infected cells, we constructed recombinant plasmids expressing fluorescent protein-tagged histone H4 molecules and visualized the intracellular localization of chromatin by their transient expression in live infected cells. Similar to other DNA viruses, the baculovirus Bombyx mori nucleopolyhedrovirus induced marginal relocation of chromatin within the nuclei of BmN cells, simultaneously with expansion of the viral DNA replication compartment, the virogenic stroma (VS). In the late stage of infection, however, the peristromal region (PR), another virus-induced subnuclear compartment, was also excluded from the chromatin-localizing area. Provided that late-gene products such as PR proteins (e.g., envelope proteins of the occlusion-derived virus) were expressed, blockage of viral DNA synthesis failed to inhibit chromatin relocation, despite abrogation of VS expansion. Instead, chromatin became marginalized concomitantly with PR expansion, suggesting that the PR contributes directly to chromatin replacement. In addition, chromatin was excluded from relatively large subnuclear structures that were induced in uninfected cells by cotransfection with four baculovirus genes, ie1, lef3, p143, and hr. Omission of any of the four genes, however, failed to result in formation of the large structures or chromatin exclusion. This correlation between compartmentalization and chromatin exclusion suggests the possibility that a chromatin-exclusive property of viral molecules, at least in part, supports nuclear compartmentalization of virus-infected cells.
Authors:
Toshihiro Nagamine; Yu Kawasaki; Atsushi Abe; Shogo Matsumoto
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-23
Journal Detail:
Title:  Journal of virology     Volume:  82     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-11     Completed Date:  2008-08-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6409-18     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Entomology, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. tnaga@riken.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Bombyx / virology*
Chromatin Assembly and Disassembly / physiology*
DNA Primers / genetics
DNA Replication / physiology*
Genes, Viral / genetics
In Situ Hybridization, Fluorescence
Nucleopolyhedrovirus / physiology*
Plasmids / genetics
Transfection
Chemical
Reg. No./Substance:
0/DNA Primers
Comments/Corrections

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