Document Detail


Nuclear magnetic resonance spectroscopy to determine the micellar cholesterol in human bile.
MedLine Citation:
PMID:  1547885     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cholesterol of gallstones comes from the vesicular rather than the micellar phase of bile. Progress in this field has been limited because conventional analytical methods disturb the distribution of cholesterol between the two phases. The resonance of the cholesterol C6 proton occurs at a chemical shift of 5.4 ppm, to be shown by 2D NMR to be specific for biliary cholesterol, and arises only from the micellar mode. Thus integration of the C6 proton resonance peak area provides a direct non-invasive determination of the cholesterol distribution in human bile.
Authors:
J P Ellul; G M Murphy; H G Parkes; R Z Slapa; R H Dowling
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  300     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-04-20     Completed Date:  1992-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  30-2     Citation Subset:  IM    
Affiliation:
Gastroenterology Unit, United Medical School of Guy's Hospital, London, UK.
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MeSH Terms
Descriptor/Qualifier:
Bile / chemistry*
Cholesterol / chemistry*
Humans
Magnetic Resonance Spectroscopy
Micelles*
Chemical
Reg. No./Substance:
0/Micelles; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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