Document Detail


Nuclear magnetic resonance line-shape analysis of fluorine-19-labeled phospholipids.
MedLine Citation:
PMID:  6897614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The application of a fluorine-19 probe to the problem of motions present in the hydrophobic region of phospholipid dispersions and biological membranes has been extended to the study of phospholipids labeled with fluorine-19 in the 8 position and with deuterium in the 2, 7, and 9 positions of the 2-acyl chain. 1-Myristoyl-2-(8,8-difluoro[2,2,7,7,9,9-2H6]myristoyl)-sn-glycero-3- phosphocholine and its nondeuterated analogue have been investigated by 19F nuclear magnetic resonance at 282.4 MHz. Spectra obtained from macroscopically oriented bilayers exhibit Pake doublets from which order parameters can be obtained. The spectra obtained from nonoriented liposomes of the phospholipids can be explained in a satisfactory manner as a random superposition of doublets broadened by heteronuclear magnetic dipole-dipole interactions. From an analysis of the data, several conclusions about the motional state of the hydrocarbon chains in the liquid-crystalline phase can be drawn. The present results show that appropriate fluorine-19 probes in the acyl chains of phospholipids can be used to investigate the structure and dynamics of model and biological membranes.
Authors:
M Engelsberg; S R Dowd; V Simplaceanu; B W Cook; C Ho
Related Documents :
15455064 - Chemical and physical analyses of wax ester properties.
12207704 - Mechanism of membrane fluidity optimization: isothermal control of the bacillus subtili...
8193124 - Regulation and phase equilibria of membrane lipids from bacillus megaterium and acholep...
190104 - Lipid-protein interactions between human apolipoprotein a-i and defined sphingomyelin s...
17645354 - Mechanistic studies of the allylic rearrangements of alpha-silyloxy allylic silanes to ...
15903434 - Glassy behavior of a percolative water-protein system.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  21     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-04-21     Completed Date:  1983-04-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6985-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chemical Phenomena
Chemistry
Fluorine*
Lipid Bilayers
Magnetic Resonance Spectroscopy
Mathematics
Phospholipids*
Pulmonary Surfactants
Grant Support
ID/Acronym/Agency:
GM-26874/GM/NIGMS NIH HHS; HL-24525/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Lipid Bilayers; 0/Phospholipids; 0/Pulmonary Surfactants; 7782-41-4/Fluorine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Control of storage-protein synthesis during seed development in pea (Pisum sativum L.).
Next Document:  Calcium-promoted resonance energy transfer between fluorescently labeled proteins during aggregation...