Document Detail


Nuclear magnetic resonance-based metabonomic study of early time point laminitis in an oligofructose-overload model.
MedLine Citation:
PMID:  21496096     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Reasons for performing study: NMR-metabonomics is an unbiased evaluation method, which allows to comprehensively study changes of the equine metabolic profile in early time point laminitis. This might give insight into the early stages of disease development. Objectives: To detect hitherto unknown changes in blood metabolites during the development of oligofructose-induced laminitis by comparing pre- and post induction blood samples. Methods: Prior to laminitis induction blood was sampled to establish control values. Post oligofructose administration (POA) blood was collected every 3 h for 24 h. One-dimensional (1) H-NMR spectra of the blood plasma were statistically analysed. Results: NMR resonances of >20 metabolites were identified using this technique. Already known changes (e.g. lactate concentrations) were confirmed using this method. Interestingly, oligofructose, a carbohydrate usually considered indigestible in the small intestine, or derivatives of oligofructose, was detected in plasma. Horses also showed increased phosphatidylcholine and/or low density lipoprotein levels POA, indicating a change in blood lipid composition. An increase in phosphatidylcholine is consistent with the breakdown of the mucosal layer of the large intestine and increased permeability of the gut. Conclusion: Due to the nontargeted approach of metabonomics, new unexpected changes can be identified, in this case the hitherto unknown oligofructose uptake through the mucosal wall and the phospholipid changes. Potential relevance: Metabolic changes in disease can be observed using NMR metabonomics. Oligofructose is used in feedstuffs and transport mechanisms through the mucosa should be studied. Phospholipids could point to a compromise of the intestinal wall during laminitis development.
Authors:
M D Keller; C C Pollitt; U C Marx
Related Documents :
887806 - Effect of prostaglandins f2alpha and e2 on milk ejection, blood pressure and blood flow...
6651686 - Acute poisoning in cattle after eating old non-viable seed of chou moellier (brassica o...
15598236 - Vaginal and cervical dilatation intrapartum is associated with eosinophilic infiltratio...
8066016 - Antioxidant status in the blood of patients with active vitiligo.
7912206 - Prediction of intracerebral haemorrhage after carotid endarterectomy by clinical criter...
23258036 - High-speed interferometric spectrally encoded flow cytometry.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-15
Journal Detail:
Title:  Equine veterinary journal     Volume:  -     ISSN:  0425-1644     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-4-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0173320     Medline TA:  Equine Vet J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 EVJ Ltd.
Affiliation:
The University of Queensland, Australian Equine Laminitis Research Unit, School of Veterinary Science Centre for Advanced Imaging Institute for Molecular Bioscience, SRC for Functional and Applied Genomics.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The difference in kinematics of horses walking, trotting and cantering on a flat and banked 10 m cir...
Next Document:  Morphological spectrum of primary epidermal laminae in the forehoof of Thoroughbred horses.