Document Detail

Nuclear location of synthetic oligonucleotides microinjected somatic cells: its implication in an antisense strategy.
MedLine Citation:
PMID:  1668686     Owner:  NLM     Status:  MEDLINE    
Synthetic oligonucleotides are widely used to modulate gene expression. However their development as genetic tools and as molecule of therapeutic interest is restricted by the poor knowledge of their mechanism of action and of their uptake by cells. We recently found that microinjected oligonucleotides accumulates in the nucleus of the cells. These observations are described and their implications are discussed.
N Mechti; J P Leonetti; J P Clarenc; G Degols; B Lebleu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids symposium series     Volume:  -     ISSN:  0261-3166     ISO Abbreviation:  Nucleic Acids Symp. Ser.     Publication Date:  1991  
Date Detail:
Created Date:  1992-11-25     Completed Date:  1992-11-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8007206     Medline TA:  Nucleic Acids Symp Ser     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  147-50     Citation Subset:  IM    
Laboratoire de Biochimie des Proteines, UA CNRS 1191, Université de Montpellier II, Montpellier, France.
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MeSH Terms
Cell Nucleus / metabolism*
Culture Media, Serum-Free
Fibroblasts / metabolism
Oligonucleotides / chemical synthesis,  metabolism*
Oligonucleotides, Antisense / chemical synthesis,  metabolism*
RNA, Messenger / genetics
RNA, Small Nuclear / metabolism
Ribonucleoproteins, Small Nuclear / metabolism
Vesicular stomatitis Indiana virus / genetics
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Oligonucleotides; 0/Oligonucleotides, Antisense; 0/RNA, Messenger; 0/RNA, Small Nuclear; 0/Ribonucleoproteins, Small Nuclear

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