Document Detail

Nuclear location-dependent role of peripheral benzodiazepine receptor (PBR) in hepatic tumoral cell lines proliferation.
MedLine Citation:
PMID:  15769477     Owner:  NLM     Status:  MEDLINE    
PBR is involved in numerous biological functions, including steroid biosynthesis, mitochondrial oxidative phosphorylation and cell proliferation. The presence of PBR at the perinuclear/nuclear subcellular level has been demonstrated in aggressive breast cancer cell lines and human glioma cells where it seems to be involved in cell proliferation. In our study we investigated the presence of perinuclear/nuclear PBR in different hepatic tumor cell lines with regard to binding to [3H] PK 11195 and protein analysis. The results obtained by saturation binding experiments and scatchard analysis of perinuclear/nuclear PBR density in parallel with the results on the growth curves of the cell lines tested, indicate that the perinuclear/nuclear PBR density correlates inversely with cell doubling time. Moreover, the cell line with high perinuclear/nuclear PBR proliferated in response to PBR ligand, whereas that with low perinuclear/nuclear PBR did not. Our results reinforce the idea that the subcellular localisation of PBR defines its function and that this receptor could be a possible target for new strategies against cancer.
L Corsi; E Geminiani; R Avallone; M Baraldi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  76     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-16     Completed Date:  2005-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  2523-33     Citation Subset:  IM    
Dept. of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Campi 183, 41100, Modena, Italy.
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MeSH Terms
Active Transport, Cell Nucleus / physiology
Antineoplastic Agents / pharmacology
Carcinoma, Hepatocellular / metabolism*,  pathology
Cell Line, Tumor
Cell Nucleus / metabolism*
Cell Proliferation* / drug effects
Cytoplasm / metabolism
Isoquinolines / pharmacology
Liver Neoplasms / metabolism*,  pathology
Liver Neoplasms, Experimental / metabolism*,  pathology
Rats, Wistar
Receptors, GABA-A / metabolism*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Isoquinolines; 0/Receptors, GABA-A; 85340-56-3/PK 11195

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