| Nuclear localization of a novel human syntaxin 1B isoform. | |
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MedLine Citation:
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PMID: 18691641 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The syntaxins are proteins associated with various intracellular membrane compartments. They are major participants in a large variety of physiological processes where membrane fusion occurs, including exocytosis. We have identified a novel syntaxin isoform generated by alternative splicing of the human STX1B gene. In contrast with the canonical syntaxins, this isoform (STX1B-DeltaTMD) lacked the classical C-terminal transmembrane domain and localized to the nucleus of various tumoral and non-tumoral cell types including human brain cortical neurons in vivo. The reversible blockade of STX1B-DeltaTMD nuclear import demonstrated that nuclear import occurred via a Ran-dependent pathway. A specific and glycine-rich C-terminus of 15 amino acids served as an unconventional nuclear localization signal. STX1B-DeltaTMD colocalized with Lamin A/C and NuMA (NUclear Mitotic Apparatus protein) in interphasic nuclei, and with NuMA and gamma-tubulin in the pericentrosomal region of the mitotic spindle in dividing cells. In a series of 37 human primary brain tumors, the ratio of STX1B-DeltaTMD to Lamin A/C transcripts was a significant prognostic marker of survival, independent of tumor staging. The characterization of STX1B-DeltaTMD as the first nucleoplasmic syntaxin with no transmembrane domain, illustrates the importance of alternative splicing in the emergence of unsuspected properties of the syntaxins in human cells, in both physiological and pathological conditions. |
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Authors:
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Sandrine Pereira; Annick Massacrier; Patrice Roll; Alain Vérine; Marie-Christine Etienne-Grimaldi; Yannick Poitelon; Andrée Robaglia-Schlupp; Sarah Jamali; Nathalie Roeckel-Trevisiol; Barbara Royer; Pierre Pontarotti; Christian Lévêque; Michael Seagar; Nicolas Lévy; Pierre Cau; Pierre Szepetowski |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-17 |
Journal Detail:
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Title: Gene Volume: 423 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-09-15 Completed Date: 2008-11-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: Netherlands |
Other Details:
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Languages: eng Pagination: 160-71 Citation Subset: IM |
Affiliation:
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INSERM UMR 910, Université de la Méditerranée, Faculté de Médecine, 13385 Marseille Cedex 5, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing
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genetics Brain Neoplasms / pathology Cell Line, Tumor Cell Nucleus / metabolism* Cell Survival Centrosome / metabolism Gene Expression Regulation, Neoplastic Humans Lamin Type A / genetics Mutant Proteins / metabolism Nuclear Matrix / metabolism Protein Isoforms / chemistry, metabolism Protein Structure, Tertiary Protein Transport RNA, Messenger / genetics, metabolism Syntaxin 1 / chemistry, metabolism* ran GTP-Binding Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Lamin Type A; 0/Mutant Proteins; 0/Protein Isoforms; 0/RNA, Messenger; 0/Syntaxin 1; EC 3.6.5.2/ran GTP-Binding Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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