Document Detail

Nuclear localization of a novel human syntaxin 1B isoform.
MedLine Citation:
PMID:  18691641     Owner:  NLM     Status:  MEDLINE    
The syntaxins are proteins associated with various intracellular membrane compartments. They are major participants in a large variety of physiological processes where membrane fusion occurs, including exocytosis. We have identified a novel syntaxin isoform generated by alternative splicing of the human STX1B gene. In contrast with the canonical syntaxins, this isoform (STX1B-DeltaTMD) lacked the classical C-terminal transmembrane domain and localized to the nucleus of various tumoral and non-tumoral cell types including human brain cortical neurons in vivo. The reversible blockade of STX1B-DeltaTMD nuclear import demonstrated that nuclear import occurred via a Ran-dependent pathway. A specific and glycine-rich C-terminus of 15 amino acids served as an unconventional nuclear localization signal. STX1B-DeltaTMD colocalized with Lamin A/C and NuMA (NUclear Mitotic Apparatus protein) in interphasic nuclei, and with NuMA and gamma-tubulin in the pericentrosomal region of the mitotic spindle in dividing cells. In a series of 37 human primary brain tumors, the ratio of STX1B-DeltaTMD to Lamin A/C transcripts was a significant prognostic marker of survival, independent of tumor staging. The characterization of STX1B-DeltaTMD as the first nucleoplasmic syntaxin with no transmembrane domain, illustrates the importance of alternative splicing in the emergence of unsuspected properties of the syntaxins in human cells, in both physiological and pathological conditions.
Sandrine Pereira; Annick Massacrier; Patrice Roll; Alain Vérine; Marie-Christine Etienne-Grimaldi; Yannick Poitelon; Andrée Robaglia-Schlupp; Sarah Jamali; Nathalie Roeckel-Trevisiol; Barbara Royer; Pierre Pontarotti; Christian Lévêque; Michael Seagar; Nicolas Lévy; Pierre Cau; Pierre Szepetowski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-17
Journal Detail:
Title:  Gene     Volume:  423     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-09-15     Completed Date:  2008-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  160-71     Citation Subset:  IM    
INSERM UMR 910, Université de la Méditerranée, Faculté de Médecine, 13385 Marseille Cedex 5, France.
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MeSH Terms
Alternative Splicing / genetics
Brain Neoplasms / pathology
Cell Line, Tumor
Cell Nucleus / metabolism*
Cell Survival
Centrosome / metabolism
Gene Expression Regulation, Neoplastic
Lamin Type A / genetics
Mutant Proteins / metabolism
Nuclear Matrix / metabolism
Protein Isoforms / chemistry,  metabolism
Protein Structure, Tertiary
Protein Transport
RNA, Messenger / genetics,  metabolism
Syntaxin 1 / chemistry,  metabolism*
ran GTP-Binding Protein / metabolism
Reg. No./Substance:
0/Lamin Type A; 0/Mutant Proteins; 0/Protein Isoforms; 0/RNA, Messenger; 0/Syntaxin 1; EC GTP-Binding Protein

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