Document Detail


Nuclear localization of the metastasis-related protein S100A4 correlates with tumour stage in colorectal cancer.
MedLine Citation:
PMID:  12898594     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A large number of experimental studies have linked the S100A4 gene product to the metastatic phenotype of cancer cells and clinical evidence indicates a correlation between S100A4 expression and poor prognosis in several cancer types. The aim of the present study was to analyse the expression of the S100A4 protein in colorectal cancer. Paraffin-embedded samples from 277 colorectal cancer patients were immunostained with anti-S100A4 antibody. Cytoplasmic staining was observed in 178 of 277 samples (64%), whereas, unexpectedly, nuclear expression of S100A4 was found in 88 of 277 of the samples (32%). This novel finding was confirmed by western blot analysis of nuclear fractions isolated from frozen tumour tissue. Statistical analysis revealed a significant correlation between nuclear expression of S100A4 and tumour stage at diagnosis, while there was no such correlation between cytoplasmic staining and tumour stage. The nuclear localization of S100A4 in colorectal cancer and its relationship to tumour stage suggest that this protein may be involved in gene regulatory pathways of relevance to the metastatic phenotype of cancer cells.
Authors:
Kjersti Flatmark; Kjetil Boye Pedersen; Jahn M Nesland; Heidi Rasmussen; Geir Aamodt; Svein-Ole Mikalsen; Kristin Bjørnland; Øystein Fodstad; Gunhild M Maelandsmo
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Publication Detail:
Type:  Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pathology     Volume:  200     ISSN:  0022-3417     ISO Abbreviation:  J. Pathol.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-08-04     Completed Date:  2003-10-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204634     Medline TA:  J Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  589-95     Citation Subset:  IM    
Copyright Information:
Copyright 2003 John Wiley & Sons, Ltd.
Affiliation:
Department of Tumor Biology and Institute for Cancer Research, The Norwegian Radium Hospital, 0310 Oslo, Norway.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / metabolism*,  pathology,  secondary
Adult
Aged
Aged, 80 and over
Blotting, Western
Cell Nucleus / metabolism
Colorectal Neoplasms / metabolism*,  pathology
Cryopreservation
Cytoplasm / metabolism
Female
Humans
Immunoenzyme Techniques
Male
Middle Aged
Neoplasm Proteins / metabolism*
Neoplasm Staging
Prospective Studies
S100 Proteins / metabolism*
Tumor Markers, Biological / metabolism*
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/S100 Proteins; 0/Tumor Markers, Biological; 142662-27-9/S100A4 protein, human

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