| Nuclear localization mechanism of Hsp105beta and its possible function in mammalian cells. | |
| | |
MedLine Citation:
|
PMID: 19028714 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Hsp105alpha and Hsp105beta are mammalian stress proteins of the Hsp105/110 family. We have shown that Hsp105beta localizes to the nucleus, whereas Hsp105alpha localizes to the cytoplasm of mammalian cells. Hsp105alpha localizes in the cytoplasm, as the nuclear export signal (NES) activity rather than nuclear localization signal (NLS) activity dominates in Hsp105alpha, due to suppression of the NLS activity. In this study, we determined the mechanisms behind the nuclear localization of Hsp105beta, and revealed that the NES was suppressed by the N-terminal (amino acids 3-10) or C-terminal (amino acids 699-756) region of Hsp105beta, and the NLS activity rather than NES activity seemed to dominate in Hsp105beta. Furthermore, as Hsp105beta which localizes in the nucleus, functioned as an inducer of Hsp70 in mammalian cells, Hsp105 family proteins may play an important role in the protection of cells against deleterious stressor together with Hsp70. |
| | |
Authors:
|
Youhei Saito; Nobuyuki Yamagishi; Takumi Hatayama |
Related Documents
:
|
11504384 - Heterogeneity of caprine kappa-casein macropeptide. 2090104 - Toxic impact of phosphamidon on acid phosphatase of prawn, metapenaeus monoceros (fabri... 765744 - Genes coding for the structure of the acid phosphatases in saccharomyces cerevisiae. 4968524 - Digestion and the distribution of acid phosphatase in blepharisma. 6773944 - Cleavage of pyrogallol by non-heme iron-containing dioxygenases. 8162174 - Drug competition for intracellular triiodothyronine-binding sites. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-20 |
Journal Detail:
|
Title: Journal of biochemistry Volume: 145 ISSN: 1756-2651 ISO Abbreviation: J. Biochem. Publication Date: 2009 Feb |
Date Detail:
|
Created Date: 2009-01-23 Completed Date: 2009-07-15 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376600 Medline TA: J Biochem Country: Japan |
Other Details:
|
Languages: eng Pagination: 185-91 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular Biology, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals COS Cells Cell Nucleus / metabolism* Cercopithecus aethiops Cytoplasm / metabolism Fluorescent Antibody Technique HSC70 Heat-Shock Proteins / metabolism HSP110 Heat-Shock Proteins / analysis*, genetics, metabolism* Mice Nuclear Localization Signals / metabolism Transfection |
| Chemical | |
Reg. No./Substance:
|
0/HSC70 Heat-Shock Proteins; 0/HSP110 Heat-Shock Proteins; 0/Hsp105 protein, mouse; 0/Nuclear Localization Signals |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Successful catheter ablation to accessory atrioventricular pathway as cardiac resynchronization ther...
Next Document: Brief report: a qualitative analysis of discussions about HIV in families of parents with HIV.