| Nuclear localization of active matrix metalloproteinase-2 in cigarette smoke-exposed apoptotic endothelial cells. | |
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MedLine Citation:
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PMID: 19191105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cigarette smoke (CS)-induced activation of proteases such as matrix metalloproteinases (MMPs) contributes to lung alveolar destruction due to cell death. The aim of this study was to determine whether MMPs are produced in pulmonary artery endothelial cells (PAECs) and whether CS activation of MMPs is associated with apoptosis. Cultured PAECs were exposed to CS and subjected to assessments of apoptosis and MMPs. Western blotting and in situ zymography were performed to localize gelatinolytic activity and to identify enzymes. CS-induced apoptosis, i.e., enhanced annexin V binding and cleaved poly-ADP-ribose-polymerase (PARP), correlated with increased degradation of gelatin, a substrate of MMPs. The levels of pro-MMP-2 and active MMP-2 were increased in cytosolic and nuclear fractions isolated from CS-exposed cells. MMP-2 protein colocalized with gelatinolytic activity in the nucleus of CS-exposed cells undergoing apoptosis. These observations support the notion that MMP-2 contributes to CS-induced gelatinase activity, which localizes in the nuclear region primarily and correlates with annexin V binding and PARP cleavage. This suggests a novel function of MMP-2 in the degradation of the nuclear matrix in CS-induced endothelial apoptosis. |
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Authors:
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Ruta Aldonyte; Aldonyte Ruta; Mark Brantly; Brantly Mark; Edward Block; Block Edward; Jawaharlal Patel; Patel Jawaharlal; Jianliang Zhang; Zhang Jianliang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental lung research Volume: 35 ISSN: 1521-0499 ISO Abbreviation: Exp. Lung Res. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-02-04 Completed Date: 2009-03-12 Revised Date: 2010-02-22 |
Medline Journal Info:
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Nlm Unique ID: 8004944 Medline TA: Exp Lung Res Country: United States |
Other Details:
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Languages: eng Pagination: 59-75 Citation Subset: IM |
Affiliation:
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Department of Medicine, Division of Pulmonary Medicine, University of Florida, Gainesville, Florida 32610, USA. aldonyte.ruta@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Annexin A5 / metabolism Apoptosis / drug effects* Cell Nucleus / drug effects*, enzymology, pathology Cells, Cultured Endothelium, Vascular / drug effects*, enzymology, pathology Matrix Metalloproteinase 2 / metabolism* Poly(ADP-ribose) Polymerases / metabolism Pulmonary Artery / drug effects*, enzymology, pathology Smoke / adverse effects Smoking / adverse effects Swine |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/Smoke; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.24.24/Matrix Metalloproteinase 2 |
| Comments/Corrections | |
Erratum In:
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Exp Lung Res. 2009 Dec;35(10):896 Note: Ruta, Aldonyte [corrected to Aldonyte, Ruta]; Mark, Brantly [corrected to Brantly, Mark]; Edward, Block [corrected to Block, Edward]; Jawaharlal, Patel [corrected to Patel, Jawaharlal]; Jianliang, Zhang [corrected to Zhang, Jianliang] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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