Document Detail

Nuclear iron deposits in hepatocytes of iron-loaded HFE-knock-out mice: a morphometric and immunocytochemical analysis.
MedLine Citation:
PMID:  15866286     Owner:  NLM     Status:  MEDLINE    
Nuclear deposits of stainable iron in hepatocytes are a sign of liver iron overload in mice. Animals with no, partial or total knock-out of the HFE alleles, the deletion of which is responsible for hereditary haemochromatosis, were given different forms of dietary iron to measure nuclear iron deposits which were then related to cytoplasmic iron load. Wild type and heterozygous HFE-knock-out mice kept for 52 weeks on a standard diet showed no such deposits. These were, however, demonstrated in low numbers and with small diameters in homozygous HFE-knock-out mice kept on this diet. Nuclear iron deposits were most abundant in all type of mice fed carbonyl iron (2.5% w/w) for 52 weeks almost irrespective of their genetic background. The diameter of these deposits increased with the genetically conditioned extent of hepatocellular iron overload. Mice that were fed a diet containing TMH-ferrocene for 4 weeks showed amounts of hepatic iron that were comparable to those in the carbonyl iron-fed group but nuclear deposits were small and present in only 0.3% of the hepatocytes. While surrounding karyoplasm was immunostained for H- and L-ferritin, the nuclear iron deposits were not. As the nuclear iron deposits corresponded electron microscopically to aggregated ferritin molecules, they represent a non-immunoreactive form of presumably denatured ferritin.
Björn Magens; Jochen Düllmann; Klaus Schümann; Uwe Wulfhekel; Peter Nielsen
Related Documents :
18437096 - The effect of t0901317 on atp-binding cassette transporter a1 and niemann-pick type c1 ...
20398226 - Plasma lipoproteins in visceral leishmaniasis and their effect on leishmania-infected m...
17219776 - Heritable, diet-induced hyperlipidemia in california mice (peromyscus californicus) is ...
11696576 - Monocyte/macrophage expression of abca1 has minimal contribution to plasma hdl levels.
24278546 - Inhalation exposure to nickel hydroxide nanoparticles induces systemic acute phase resp...
18761006 - Cryopreserved peripheral blood mononuclear cells are suitable for the assessment of imm...
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2005-03-17
Journal Detail:
Title:  Acta histochemica     Volume:  107     ISSN:  0065-1281     ISO Abbreviation:  Acta Histochem.     Publication Date:  2005  
Date Detail:
Created Date:  2005-05-03     Completed Date:  2005-09-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  57-65     Citation Subset:  IM    
Institute of Anatomy II: Experimental Morphology, University Hospital Hamburg-Eppendorf, Martinistrasse 52, Hamburg 20246, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Nucleus / chemistry*,  metabolism,  pathology
Hemochromatosis / genetics,  metabolism,  pathology*
Hepatocytes / chemistry*,  metabolism,  ultrastructure
Histocompatibility Antigens Class I / genetics*
Iron / administration & dosage,  analysis*,  metabolism
Iron Overload / metabolism,  pathology*
Membrane Proteins / genetics*
Mice, Inbred C57BL
Mice, Knockout
Reg. No./Substance:
0/Hfe protein, mouse; 0/Histocompatibility Antigens Class I; 0/Membrane Proteins; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Localization of sulfated sialic acids in the dentinal tubules during tooth formation in mice.
Next Document:  Effects of electroacupuncture with different frequencies on spinal ionotropic glutamate receptor exp...