| Nuclear factors involved in mitochondrial translation cause a subgroup of combined respiratory chain deficiency. | |
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MedLine Citation:
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PMID: 21169334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mutations in several mitochondrial DNA and nuclear genes involved in mitochondrial protein synthesis have recently been reported in combined respiratory chain deficiency, indicating a generalized defect in mitochondrial translation. However, the number of patients with pathogenic mutations is small, implying that nuclear defects of mitochondrial translation are either underdiagnosed or intrauterine lethal. No comprehensive studies have been reported on large cohorts of patients with combined respiratory chain deficiency addressing the role of nuclear genes affecting mitochondrial protein synthesis to date. We investigated a cohort of 52 patients with combined respiratory chain deficiency without causative mitochondrial DNA mutations, rearrangements or depletion, to determine whether a defect in mitochondrial translation defines the pathomechanism of their clinical disease. We followed a combined approach of sequencing known nuclear genes involved in mitochondrial protein synthesis (EFG1, EFTu, EFTs, MRPS16, TRMU), as well as performing in vitro functional studies in 22 patient cell lines. The majority of our patients were children (<15 years), with an early onset of symptoms <1 year of age (65%). The most frequent clinical presentation was mitochondrial encephalomyopathy (63%); however, a number of patients showed cardiomyopathy (33%), isolated myopathy (15%) or hepatopathy (13%). Genomic sequencing revealed compound heterozygous mutations in the mitochondrial transfer ribonucleic acid modifying factor (TRMU) in a single patient only, presenting with early onset, reversible liver disease. No pathogenic mutation was detected in any of the remaining 51 patients in the other genes analysed. In vivo labelling of mitochondrial polypeptides in 22 patient cell lines showed overall (three patients) or selective (four patients) defects of mitochondrial translation. Immunoblotting for mitochondrial proteins revealed decreased steady state levels of proteins in some patients, but normal or increased levels in others, indicating a possible compensatory mechanism. In summary, candidate gene sequencing in this group of patients has a very low detection rate (1/52), although in vivo labelling of mitochondrial translation in 22 patient cell lines indicate that a nuclear defect affecting mitochondrial protein synthesis is responsible for about one-third of combined respiratory chain deficiencies (7/22). In the remaining patients, the impaired respiratory chain activity is most likely the consequence of several different events downstream of mitochondrial translation. Clinical classification of patients with biochemical analysis, genetic testing and, more importantly, in vivo labelling and immunoblotting of mitochondrial proteins show incoherent results, but a systematic review of these data in more patients may reveal underlying mechanisms, and facilitate the identification of novel factors involved in combined respiratory chain deficiency. |
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Authors:
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John P Kemp; Paul M Smith; Angela Pyle; Vivienne C M Neeve; Helen A L Tuppen; Ulrike Schara; Beril Talim; Haluk Topaloglu; Elke Holinski-Feder; Angela Abicht; Birgit Czermin; Hanns Lochmüller; Robert McFarland; Patrick F Chinnery; Zofia M A Chrzanowska-Lightowlers; Robert N Lightowlers; Robert W Taylor; Rita Horvath |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-12-17 |
Journal Detail:
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Title: Brain : a journal of neurology Volume: 134 ISSN: 1460-2156 ISO Abbreviation: Brain Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-27 Completed Date: 2011-01-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372537 Medline TA: Brain Country: England |
Other Details:
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Languages: eng Pagination: 183-95 Citation Subset: AIM; IM |
Affiliation:
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Mitochondrial Research Group, Institute of Human Genetics, Newcastle University, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Blotting, Western Cell Line Cell Nucleus / genetics*, metabolism Child Child, Preschool DNA, Mitochondrial / genetics, metabolism Electron Transport / genetics Female Genotype Humans Infant Male Middle Aged Mitochondria / genetics*, metabolism Mitochondrial Diseases / genetics*, metabolism, pathology Muscle, Skeletal / metabolism, pathology* Mutation Protein Biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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074454//Wellcome Trust; 074454/Z/04/Z//Wellcome Trust; BB/F011520/1//Biotechnology and Biological Sciences Research Council; G0700718//Medical Research Council |
| Chemical | |
Reg. No./Substance:
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0/DNA, Mitochondrial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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