| Nuclear estrogen receptor-mediated Notch signaling and GPR30-mediated PI3K/AKT signaling in the regulation of endometrial cancer cell proliferation. | |
| | |
MedLine Citation:
|
PMID: 22075757 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
To elucidate the mechanisms of nuclear estrogen receptor (ER)-mediated and G protein-coupled receptor 30 (GPR30)-mediated signaling in the regulation of proliferation in ER-positive and ER-negative endometrial cancer cells, two human endometrial carcinoma cell lines, Ishikawa (ER-positive) and KLE (ER-negative), were used. PCR and Western blot analyses were used to determine the effects of estrogen stimulation on the activation of Notch and GPR30-PI3K/AKT signaling. Cell growth was investigated using MTT assays. Overexpression of ER in ER-negative cells was achieved by plasmid transfection and was used to investigate the effects on cellular growth and Notch signaling. GPR30-mediated signaling was evaluated using siRNA interference. Estrogen stimulated cell proliferation in both cell lines, it activated Notch signaling in ER-positive Ishikawa cells, but not in ER-negative KLE cells. Blockade of this signaling by a Notch inhibitor resulted in partial arrest of estrogen-induced cell proliferation in Ishikawa cells. Overexpression of ER in KLE cells restored estrogen-enhanced Notch signaling and further promoted cell growth. GPR30, as a new G-protein-coupled estrogen receptor, was detected in both cell lines, but was stronger in ER-negative KLE cells. Depletion of GPR30 in KLE cells abolished estrogen-induced PI3K/AKT signaling activation and resulted in inhibition of cell proliferation. Conclusively, regulation of proliferation in nuclear ER-positive endometrial cancer cells is mediated by both ER-Notch signaling and GPR30-PI3K/AKT signaling, whereas only the latter pathway is involved in the regulation of growth in nuclear ER-negative endometrial cancer cells. |
| | |
Authors:
|
Yanan Wei; Zhenbo Zhang; Hong Liao; Ling Wu; Xiaomei Wu; Dongmei Zhou; Xiaowei Xi; Yaping Zhu; Youji Feng |
Related Documents
:
|
21637587 - Micronuclei induced by reverse transcriptase inhibitors in mononucleated and binucleate... 22210727 - Paneth cells and goblet cells express the neuroendocrine peptide synaptophysin. i- norm... 554567 - Microelectrophoretic application of metabolic modifiers of the phosphofructokinase-hexo... 21945897 - Self-folding immunoprotective cell encapsulation devices. 22510557 - Peptidyl-prolyl isomerization for cytokinetic abscission: fold and cut. 21063937 - Current and future status of stem cell therapy in heart failure. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-08 |
Journal Detail:
|
Title: Oncology reports Volume: - ISSN: 1791-2431 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9422756 Medline TA: Oncol Rep Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, P.R. China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Defining the Role of Laboratory Genetic Counselor.
Next Document: Ripe fruit of Rubus coreanus inhibits mast cell-mediated allergic inflammation.