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Nuclear envelope-associated resumption of RNA synthesis in late mitosis of HeLa cells.
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MedLine Citation:
PMID:  4752403     Owner:  NLM     Status:  MEDLINE    
The restitution of RNA synthesis in cultures progressing from metaphase into interphase (G(1)) has been investigated in synchronized HeLa S(3) cells by using inhibitors of macro-molecular synthesis and the technique of electron microscope autoradiography. The rate of incorporation of radioactive uridine into RNA approached interphase levels in the absence of renewed protein synthesis. In contrast, maintenance of this rate in G(1) was dependent upon renewed protein synthesis. Restoration of synthesis of heterogeneous nuclear RNA occurred under conditions that inhibited production of ribosomal precursor RNA. In autoradiographs of individual cells exposed to radioactive uridine, silver grains were first detected after nuclear envelope reformation at the periphery of the chromosome mass but before chromosomal decondensation. These data are consistent with the following interpretation. Multiple RNA polymerase activities persist through mitosis and are involved in the initiation of RNA synthesis in early telophase at sites on the nuclear envelope.
T Simmons; P Heywood; L Hodge
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  59     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1973 Oct 
Date Detail:
Created Date:  1974-01-15     Completed Date:  1974-01-15     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  150-64     Citation Subset:  IM    
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MeSH Terms
Carbon Radioisotopes
Cell Nucleus / drug effects,  metabolism*
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Hela Cells / metabolism*,  physiology
Membranes / physiology
Microscopy, Electron
Neoplasm Proteins / biosynthesis
Puromycin / pharmacology
RNA, Neoplasm / biosynthesis*
RNA, Ribosomal / biosynthesis
Uridine / metabolism
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Neoplasm Proteins; 0/RNA, Neoplasm; 0/RNA, Ribosomal; 50-76-0/Dactinomycin; 53-79-2/Puromycin; 58-96-8/Uridine; 66-81-9/Cycloheximide

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1973 by The Rockefeller University Press
Received Day: 13 Month: 4 Year: 1973
Revision Received Day: 18 Month: 6 Year: 1973
Print publication date: Day: 1 Month: 10 Year: 1973
Volume: 59 Issue: 1
First Page: 150 Last Page: 164
ID: 2110910
PubMed Id: 4752403

T. Simmons
P. Heywood
L. Hodge
From the Departments of Human Genetics and Microbiology, Yale University School of Medicine, New Haven, Connecticut 06510

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