Document Detail


Nuclear dynamics during germination, conidiation, and hyphal fusion of Fusarium oxysporum.
MedLine Citation:
PMID:  20543061     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In many fungal pathogens, infection is initiated by conidial germination. Subsequent stages involve germ tube elongation, conidiation, and vegetative hyphal fusion (anastomosis). Here, we used live-cell fluorescence to study the dynamics of green fluorescent protein (GFP)- and cherry fluorescent protein (ChFP)-labeled nuclei in the plant pathogen Fusarium oxysporum. Hyphae of F. oxysporum have uninucleated cells and exhibit an acropetal nuclear pedigree, where only the nucleus in the apical compartment is mitotically active. In contrast, conidiation follows a basopetal pattern, whereby mononucleated microconidia are generated by repeated mitotic cycles of the subapical nucleus in the phialide, followed by septation and cell abscission. Vegetative hyphal fusion is preceded by directed growth of the fusion hypha toward the receptor hypha and followed by a series of postfusion nuclear events, including mitosis of the apical nucleus of the fusion hypha, migration of a daughter nucleus into the receptor hypha, and degradation of the resident nucleus. These previously unreported patterns of nuclear dynamics in F. oxysporum could be intimately related to its pathogenic lifestyle.
Authors:
M Carmen Ruiz-Roldán; Michael Köhli; M Isabel G Roncero; Peter Philippsen; Antonio Di Pietro; Eduardo A Espeso
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-11
Journal Detail:
Title:  Eukaryotic cell     Volume:  9     ISSN:  1535-9786     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-04     Completed Date:  2010-11-10     Revised Date:  2011-07-22    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1216-24     Citation Subset:  IM    
Affiliation:
Departamento de Genética, Universidad de Córdoba, Edificio Gregor Mendel, Campus de Rabanales, 14071 Córdoba, Spain. ge2rurom@uco.es
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / metabolism*
Cell Polarity
Fluorescent Dyes / metabolism
Fusarium / cytology*,  growth & development,  physiology*,  ultrastructure
Germination / physiology*
Green Fluorescent Proteins / metabolism
Histones / metabolism
Hyphae / cytology*,  growth & development,  physiology*
Lycopersicon esculentum / microbiology,  ultrastructure
Mitosis
Plant Diseases / microbiology
Plant Roots / microbiology,  ultrastructure
Spores, Fungal / cytology,  physiology*
Time Factors
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Histones; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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