| Nuclear dynamics during germination, conidiation, and hyphal fusion of Fusarium oxysporum. | |
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MedLine Citation:
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PMID: 20543061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In many fungal pathogens, infection is initiated by conidial germination. Subsequent stages involve germ tube elongation, conidiation, and vegetative hyphal fusion (anastomosis). Here, we used live-cell fluorescence to study the dynamics of green fluorescent protein (GFP)- and cherry fluorescent protein (ChFP)-labeled nuclei in the plant pathogen Fusarium oxysporum. Hyphae of F. oxysporum have uninucleated cells and exhibit an acropetal nuclear pedigree, where only the nucleus in the apical compartment is mitotically active. In contrast, conidiation follows a basopetal pattern, whereby mononucleated microconidia are generated by repeated mitotic cycles of the subapical nucleus in the phialide, followed by septation and cell abscission. Vegetative hyphal fusion is preceded by directed growth of the fusion hypha toward the receptor hypha and followed by a series of postfusion nuclear events, including mitosis of the apical nucleus of the fusion hypha, migration of a daughter nucleus into the receptor hypha, and degradation of the resident nucleus. These previously unreported patterns of nuclear dynamics in F. oxysporum could be intimately related to its pathogenic lifestyle. |
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Authors:
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M Carmen Ruiz-Roldán; Michael Köhli; M Isabel G Roncero; Peter Philippsen; Antonio Di Pietro; Eduardo A Espeso |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-11 |
Journal Detail:
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Title: Eukaryotic cell Volume: 9 ISSN: 1535-9786 ISO Abbreviation: Eukaryotic Cell Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-04 Completed Date: 2010-11-10 Revised Date: 2011-07-22 |
Medline Journal Info:
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Nlm Unique ID: 101130731 Medline TA: Eukaryot Cell Country: United States |
Other Details:
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Languages: eng Pagination: 1216-24 Citation Subset: IM |
Affiliation:
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Departamento de Genética, Universidad de Córdoba, Edificio Gregor Mendel, Campus de Rabanales, 14071 Córdoba, Spain. ge2rurom@uco.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Nucleus
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metabolism* Cell Polarity Fluorescent Dyes / metabolism Fusarium / cytology*, growth & development, physiology*, ultrastructure Germination / physiology* Green Fluorescent Proteins / metabolism Histones / metabolism Hyphae / cytology*, growth & development, physiology* Lycopersicon esculentum / microbiology, ultrastructure Mitosis Plant Diseases / microbiology Plant Roots / microbiology, ultrastructure Spores, Fungal / cytology, physiology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Histones; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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