Document Detail


Nuclear degraded sperm subpopulation is affected by poor chromatin compaction and nuclease activity.
MedLine Citation:
PMID:  24606016     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
There is an interest in the nuclear degraded sperm subpopulation because, although it is present in a low percentage in all semen samples, patient groups such as varicocele and rearranged genome carriers show high levels of these degraded spermatozoa. This study is designed with two objectives in mind: first, incubations of H2 O2 and nuclease on DTT-treated and untreated samples to show the aetiology of this subpopulation and second, assessment of the correlation between the protamine ratio and nuclear degraded spermatozoa. A very high increase in the nuclear degraded subpopulation has been found with nuclease incubation, and it is even higher when it has been merged with nuclear decompaction using DTT. Alternatively, incubation with H2 O2 with and without DTT did not show such a significant increase in nuclear degraded spermatozoa. The protamine ratio correlated with this subpopulation, showing, in patients, that poor nuclear compaction would turn the sperm susceptible to degradation. Then, the assessment of nuclear degraded spermatozoa might not be only a measure of DNA degradation but also an indicator of chromatin compaction in the spermatozoa. Different patient groups would fit this model for sperm nuclear degradation, such as varicocele patients, who show a high percentage of immature spermatozoa and nuclear degraded spermatozoa, and reorganised genome carriers, where reorganisation might also cause poor chromatin compaction on the sperm nucleus.
Authors:
J Ribas-Maynou; A García-Peiró; J Martínez-Heredia; A Fernández-Encinas; C Abad; M J Amengual; J Navarro; J Benet
Related Documents :
18419056 - Hepatosplenic fungal infection in adult patients with acute leukemia.
19651006 - Presentating phases of chronic myeloid leukaemia.
12433286 - Interleukin-7 (il-7) in patients receiving intensive chemotherapy for acute myelogenous...
18008086 - Clinical efficacy of all-trans retinoic acid for treating adult t cell leukemia.
3132806 - Hodgkin's disease of the oesophagus.
2096926 - The management of hydrothorax in continuous ambulatory peritoneal dialysis (capd).
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-10
Journal Detail:
Title:  Andrologia     Volume:  -     ISSN:  1439-0272     ISO Abbreviation:  Andrologia     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2014 Blackwell Verlag GmbH.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Enhanced glucocorticoid-induced leucine zipper in dendritic cells induces allergen-specific regulato...
Next Document:  New insights into the role of indole-3-acetic acid in the virulence of Pseudomonas savastanoi pv. sa...