Document Detail


Nuclear contrast angiography: a simple method for morphological characterization of cerebral arteries.
MedLine Citation:
PMID:  19401159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Visualization of the cerebral vascular tree is important in experimental stroke and cerebral vascular malformation research. We describe a simple method, nuclear contrast angiography, that enables simultaneous visualization of the arterial tree and cerebral endothelial cells in rodent brain whole mounts. A mixture of latex and black ink was injected into the arterial system of rodents, resulting in high contrast demarcation of the arterial tree of the brain. This method clearly differentiates arteries from veins. We applied this method to demonstrate that 14 days of unilateral carotid artery occlusion induces increases in the caliber of (1) bilateral anterior communicating arteries, (2) bilateral anterior cerebral arteries, and (3) ipsilateral proximal middle cerebral artery of the circle of Willis. Unlike other methods, this procedure selectively stains endothelial nuclei of arteries. Thus, cerebral endothelial nuclei can be visualized, quantitated, and morphologically characterized at the same time the cortical arterial tree is delineated. This method should be useful in studies of stroke and cerebral arteriogenesis, which require the accurate assessment of both arterial diameters and endothelial cell density.
Authors:
He Meng; Yezi Peng; Rushdee Hasan; Genggeng Yu; Michael M Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-01-22
Journal Detail:
Title:  Brain research     Volume:  1261     ISSN:  1872-6240     ISO Abbreviation:  Brain Res.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-10-30     Completed Date:  2010-01-14     Revised Date:  2013-05-23    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  75-81     Citation Subset:  IM    
Affiliation:
Department of Neurology, University of Michigan Medical School, Ann Arbor, MI 48109-5622, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Ischemia / physiopathology
Cell Size
Cerebral Angiography / methods*
Cerebral Arteries / anatomy & histology*,  physiology
Endothelial Cells / cytology
Female
Male
Mice
Mice, Inbred C57BL
Neovascularization, Physiologic / physiology
Rats
Rats, Sprague-Dawley
Retinal Artery / anatomy & histology,  physiology
Grant Support
ID/Acronym/Agency:
K02 NS054724-03/NS/NINDS NIH HHS; NS054724/NS/NINDS NIH HHS; R01 NS062816/NS/NINDS NIH HHS
Comments/Corrections

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