Document Detail


Nuclear angiotensin receptors induce transcription of renin and angiotensinogen mRNA.
MedLine Citation:
PMID:  8406654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The observation that nuclei from hepatic tissue exhibit specific angiotensin II (Ang II) binding led us to explore whether Ang II modulates mRNA in general, mRNA specific for renin system components, or both. Nuclei from hepatic tissue exhibited a single high-affinity (Kd = 0.4 nmol/L) Ang II-specific binding site, which was associated with increased RNA transcription. Whereas total RNA extracted from nuclei increased 1.5-fold in response to Ang II (10(-9) mol/L), specific mRNA for renin and angiotensinogen increased 7.8- and 2.5-fold, respectively. Ang II binding and induced transcription showed parallel Ang II dose responses that were both inhibited by 10(-5) mol/L DuP 753 or saralasin. Maximum Ang II binding and RNA transcription occurred at the same Ang II concentration (10(-9) mol/L). Higher doses of Ang II resulted in a progressive decrease in RNA transcription. Together, these results demonstrate that hepatic nuclei have functional Ang II-specific receptors. It is concluded that Ang II may elicit responses at nuclear receptors, which heretofore were associated only with Ang II receptors located on plasma membranes. However, the individual contribution of plasma and nuclear membrane Ang II receptors to the overall cellular Ang II transcriptional response and their possible interactions remain to be determined.
Authors:
P Eggena; J H Zhu; K Clegg; J D Barrett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hypertension     Volume:  22     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1993-11-04     Completed Date:  1993-11-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  496-501     Citation Subset:  IM    
Affiliation:
Renin Biochemistry/Vascular Pharmacology Laboratories, Department of Veterans Affairs Medical Center, Sepulveda, CA 91343.
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MeSH Terms
Descriptor/Qualifier:
Angiotensin II / metabolism,  pharmacology
Angiotensinogen / genetics*
Animals
Cell Nucleus / metabolism*
Dose-Response Relationship, Drug
Liver / metabolism
Nucleic Acid Hybridization
RNA, Messenger / genetics*,  metabolism
Rats
Rats, Wistar
Receptors, Angiotensin / physiology*
Renin / genetics*
Transcription, Genetic* / drug effects
Grant Support
ID/Acronym/Agency:
HL-42636/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Angiotensin; 11002-13-4/Angiotensinogen; 11128-99-7/Angiotensin II; EC 3.4.23.15/Renin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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