| Nuclear accumulation of epidermal growth factor in cultured rat pituitary cells. | |
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MedLine Citation:
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PMID: 6968406 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Epidermal growth factor (EGF) is a mitogen for epidermal cells in vivo and for a wide variety of cells in culture. Recently, we and others have reported that EGF can also regulate the cellular levels of various hormones and fibronectin at concentrations which only minimally influence cell division. In addition, EGF treatment of GH3 cells affects chromatin structure such that isolated nuclei from treated cells have an increased capacity to bind bacterial RNA polymerase in initiation site complexes. Thus, the data suggest that various nuclear functions are modulated by EGF in GH3 cells despite its failure to affect DNA synthesis or cell proliferation. Recently, Yanker and Shooter have reported on the nuclear accumulation of nerve growth factor (NGF) in PC12 cells in which NGF does not promote cell division but does influence RNA and protein synthesis while inducing overt differentiation (neurite outgrowth). The similarities between the two systems and the various theories regarding the mechanism by which mitogens exert their growth-promoting and other effects led us to investigate whether an interaction between EGF and the cell nucleus can be demonstrated after surface binding and internalization of EGF in GH3 cells. We report here that when its lysosomal degradation is inhibited by chloroquine, EGF accumulates in the nucleus. |
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Authors:
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L K Johnson; I Vlodavsky; J D Baxter; D Gospodarowicz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature Volume: 287 ISSN: 0028-0836 ISO Abbreviation: Nature Publication Date: 1980 Sep |
Date Detail:
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Created Date: 1980-12-18 Completed Date: 1980-12-18 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0410462 Medline TA: Nature Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 340-3 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Cell Nucleus / metabolism* Cells, Cultured Chloroquine / pharmacology Epidermal Growth Factor / metabolism* Kinetics Peptides / metabolism* Pituitary Gland / metabolism*, ultrastructure Rats |
| Chemical | |
Reg. No./Substance:
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0/Peptides; 54-05-7/Chloroquine; 62229-50-9/Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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