Document Detail


Nuclear accumulation of cyclin D1 following long-term fractionated exposures to low-dose ionizing radiation in normal human diploid cells.
MedLine Citation:
PMID:  24583467     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cyclin D1 is a mitogenic sensor that responds to growth signals from the extracellular environment and regulates the G 1-to-S cell cycle transition. When cells are acutely irradiated with a single dose of 10 Gy, cyclin D1 is degraded, causing cell cycle arrest at the G 1/S checkpoint. In contrast, cyclin D1 accumulates in human tumor cells that are exposed to long-term fractionated radiation (0.5 Gy/fraction of X-rays). In this study we investigated the effect of fractionated low-dose radiation exposure on cyclin D1 localization in 3 strains of normal human fibroblasts. To specifically examine the nuclear accumulation of cyclin D1, cells were treated with a hypotonic buffer containing detergent to remove cytoplasmic cyclin D1. Proliferating cell nuclear antigen (PCNA) immunofluorescence was used to identify cells in S phase. With this approach, we observed S-phase nuclear retention of cyclin D1 following low-dose fractionated exposures, and found that cyclin D1 nuclear retention increased with exposure time. Cells that retained nuclear cyclin D1 were more likely to have micronuclei than non-retaining cells, indicating that the accumulation of nuclear cyclin D1 was associated with genomic instability. Moreover, inhibition of the v-akt murine thymoma viral oncogene homolog (AKT) pathway facilitated cyclin D1 degradation and eliminated cyclin D1 nuclear retention in cells exposed to fractionated radiation. Thus, cyclin D1 may represent a useful marker for monitoring long-term effects associated with exposure to low levels of radiation.
Authors:
Tsutomu Shimura; Nobuyuki Hamada; Megumi Sasatani; Kenji Kamiya; Naoki Kunugita
Related Documents :
23535377 - Beta-actin variant is necessary for enterovirus 71 replication.
23421427 - Pdgf-mediated autophagy regulates vascular smooth muscle cell phenotype and resistance ...
23077177 - Na,k-atpase β-subunit cis homo-oligomerization is necessary for epithelial lumen format...
23592297 - Silk fibroin scaffolds enhance cell commitment of adult rat cardiac progenitor cells.
187247 - Cell growth and quabain-sensitive 86rg+ uptake and (na++k+)-atpase activity in 3t3 and ...
2281877 - The cellsoft computerized semen analysis system. ii. on the optimalization of running c...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-2-14
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  13     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2014 Feb 
Date Detail:
Created Date:  2014-3-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A large-area 15 nm graphene nanoribbon array patterned by a focused ion beam.
Next Document:  Exposure to Outgroup Members Criticizing Their Own Group Facilitates Intergroup Openness.