|The nuclear transcription factor CREB: involvement in addiction, deletion models and looking forward.|
|Jump to Full Text|
|PMID: 19305803 Owner: NLM Status: PubMed-not-MEDLINE|
|Addiction involves complex physiological processes, and is characterised not only by broad phenotypic and behavioural traits, but also by ongoing molecular and cellular adaptations. In recent years, increasingly effective and novel techniques have been developed to unravel the molecular implications of addiction. Increasing evidence has supported a contribution of the nuclear transcription factor CREB in the development of addiction, both in contribution to phenotype and expression in brain regions critical to various aspects of drug-seeking behaviour and drug reward. Abstracting from this, models have exploited these data by removing the CREB gene from the developing or developed mouse, to crucially determine its impact upon addiction-related processes. More recent models, however, hold greater promise in unveiling the contribution of CREB to disorders such as addiction.|
|Cameron S McPherson; Andrew J Lawrence|
Related Documents :
|18855803 - Stress, alcohol and drug interaction: an update of human research.
21939763 - Compounding of a topical drug with prospective natural surfactant-stabilized pharmaceut...
2905653 - Legal aspects of tardive dyskinesia.
|Type: Journal Article|
|Title: Current neuropharmacology Volume: 5 ISSN: 1570-159X ISO Abbreviation: Curr Neuropharmacol Publication Date: 2007 Sep|
|Created Date: 2009-03-23 Completed Date: 2010-06-11 Revised Date: 2013-05-23|
Medline Journal Info:
|Nlm Unique ID: 101157239 Medline TA: Curr Neuropharmacol Country: United Arab Emirates|
|Languages: eng Pagination: 202-12 Citation Subset: -|
|Brain Injury and Repair Group, Howard Florey Institute, University of Melbourne, Parkville, Victoria 3010, Australia.|
|APA/MLA Format Download EndNote Download BibTex|
Journal ID (nlm-ta): Curr Neuropharmacol
Journal ID (publisher-id): CN
Publisher: Bentham Science Publishers Ltd.
?2007 Bentham Science Publishers Ltd.
open-access: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/) which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received Day: 12 Month: 11 Year: 2006
Revision Received Day: 16 Month: 3 Year: 2007
Accepted Day: 29 Month: 3 Year: 2007
Print publication date: Month: 9 Year: 2007
Volume: 5 Issue: 3
First Page: 202 Last Page: 212
PubMed Id: 19305803
Publisher Id: CN-5-202
|The Nuclear Transcription Factor CREB: Involvement in Addiction, Deletion Models and Looking Forward|
|Cameron S McPherson12|
|Andrew J Lawrence12*|
1Brain Injury and Repair Group, Howard Florey Institute, University of Melbourne, Parkville, Victoria 3010, Australia
2Centre for Neuroscience, University of Melbourne, Parkville, Victoria 3010, Australia
|*Address correspondence to this author at Howard Florey Institute, University of Melbourne, Parkville, Victoria, 3010, Australia; Tel: +61 383440414; Fax: +61 393481707; E-mail: Andrew.Lawrence@florey.edu.au
The compulsive or uncontrolled use of a drug is often used to define addiction , which develops after repeated drug exposure, despite severe adverse consequences [58, 79]. It is the progression from recreational or controlled use of a drug to this unbalanced, compulsive state that is a distinguishing hallmark of addiction. The underlying pathology of addiction has been partly described by different theories, and prominently includes the opponent process theory [57, 109], an incentive salience model explaining excessive drug wanting [9, 53, 97], and the development of learned adaptations, describing drug-memory associations . Unifying these theories is a compelling, underlying proposal that the development of addiction relates to long term drug-induced neural adpatations, some of which may be manifest following even an acute drug exposure. Such adaptations are observed through behavioural testing and include dependence (where compensation for drug effects beget withdrawal-symptoms subsequent to cessation of drug intake) and tolerance (a condition where drug effects diminish subsequent to ongoing drug exposure ). In contrast, sensitization represents a phenomenon involving enhanced drug response, typically subsequent to a cycle of drug exposure and abstinence [9, 22, 53, 97]. Moreover, the contextual association between drug and environment dramatically impacts upon the development and expression of sensitization [9, 97]. It should be noted, however, that physical dependence upon a substance is not a necessary precondition for addiction. In sum, addiction has been likened to an abberent form of learning, involving stable changes responsible for long-term behavioural plasticity, manifesting as changes in behavioural response to acute or repeated drug exposure [33, 52, 104].
A recurring issue associated with rehabilitated drug addicts is their ongoing potential to relapse, which is not fully ameliorated either by long periods of abstinence nor psychological treatments. This suggests that molecular changes in the brain have been instantiated by a nominal period of drug exposure, not reversible by drug abstinence alone. Berke and Hyman  describe this process as a drug-induced usurpation of molecular mechanisms commonly involved with associative learning, which drive compulsive drug abuse and propensity to relapse. Whether molecular or synaptic alterations in neuronal communication or function are a homeostatic adjustment to drug insult or longer term plasticities contributing toward learned behaviours is not clear . Nevertheless, the end result is a complex neural change for which we have so far failed to fully characterise the substrates involved and the specific role that they play.
Shaywitz and Greenberg  make the point that, ?Understanding of the mechanisms by which extracellular stimuli induce changes in gene expression is critical for understanding how cells can adapt to environmental cues.? Thus, in order to comprehend the technical and complex nature of the behavioural response associated with drug abuse, the apposition of molecular evidence is required to help explain these changes at the cellular level and ultimately, neural systems level. An immediate imperative driving current addiction research is the identification of a molecular target which can mediate long term neuronal lability. ?FosB [73, 82] and PSD-95  alike have been recently identified as leading substrates in this regard, with modified expression observed up to four and eight weeks following drug stimulus respectively. Moreover, chronic cocaine has been associated with increases in dendritic spine density in the NAcc, but these changes do not appear long-lived enough to correspond with prolonged behavioural changes . Accordingly therefore, other potential molecular targets implicated in the development of addiction are worthy of examination.
It is widely believed that changes in gene expression underlie neural adaptations following exposure to drugs of abuse . A key mechanism for regulating gene expression is through nuclear induction of the transcription factor CREB, implicated also in various affective states, learning and memory. As indicated in Mayr and Montminy , ?At a mechanistic level, CREB is perhaps one of the best understood phosphorylation-dependent transcription factors. By comparison, relatively little is known about the physiological role of this protein in different systems.? This challenge has fallen to, and been taken up by, a surfeit of behavioural, molecular and neuropharmacological investigations, and slowly, a complex picture is emerging. A key challenge for investigators, however, is the advance from a discrete molecular and neuronal synthesis to that of a systems level, as thoroughly emphasised by Nestler [79-81]. Indeed, as Greengard  notes, with one hundred billion neurons in the brain, and each sharing approximately one thousand reciprocal connections, deducing the collective afferent and efferent neuronal colloquy is a formidable task. Appreciating the inter- and intra-communications of discrete neural systems comes from understanding their function at a molecular level.
Here, we examine recent evidence supporting the contribution of CREB as a key molecular mechanism in the development of addiction. Further to this, a variety of models are discussed, which have resolved to restrict or delete the expression of this transcription factor, attempt to explain the role CREB has played. Finally, we comment upon more recent and effective techniques for the restriction of CREB deletion in the adult brain.
The current state of addiction neurobiology is charactersing a molecular substrate which can partially explain the ongoing behavioural changes wrought by addiction, however, this was not always the case. Earlier work sought to determine which regions of the brain were critically involved in addiction, and subsequently, what pathways were consistently activated. An extensive body of data has now thoroughly demonstrated that the activation of the mesocorticolimbic dopamine system is a key mechanism inovolved in drug reward and reinforcement, a system which involves dopaminergic projections from the mesencephalon that synapse onto the nucleus accumbens, striatum, aymgdala and prefrontal cortex. Studies employing both lesioning experiments [27, 30, 37, 45, 48] and pharmacological manipulations [31, 103, 113] have collectively demonstrated the importance of such structures including the prefrontal cortex (PFC), nucleus accumbens (NAcc), dorsal striatum, hippocampus and amygdaloid nuclei in relation to drug-seeking behaviour and drug-induced plasticity. The activity of this network, and the interface of this network with other structures (e.g. hypothalamus and brain stem nuclei) is critical to long lasting molecular changes driving relapse.
Dopamine receptors are G-protein coupled receptors (GPCRs) which involve the regulation of adenylate cyclase (AC), generating the second messenger cyclic AMP (cAMP) from ATP. Indeed, it was as early as 1987 that cAMP was found to enhance CREB activation in PC-12 (pheochromocytoma cell line) nuclear extracts . This discovery heralded the identification of a molecular target regulating gene expression which could potentially explain the central development of addiction. Subsequent study investigated other intracellular signal transduction pathways culminating in the activation of CREB, as well as various paradigms of drug abuse which activated this key molecular substrate. As corticostriatal and corticotegmental glutamatergic efferents are also implicated in drug reward, calcium-activated CREB was examined, and the field of study burgeoned. Some of the intracellular signalling transduction pathways implicated in the activation of CREB are demonstrated in Fig. (1), and include G-protein, ion channel and growth factor drug receptor targets. These pathways often involve the association of a drug ligand with its respective receptor target, and a kinase phosphorylation cascade culminating in the translocation of a second messenger into the nucleus, phosphorylating CREB at Ser133 (pCREB). CREB is a member of a basic leucine zipper (bZIP) CREB/ATF-1 sub-family which includes members CREM (cAMP Responsive Element Modulatory protein) and ATF-1 (Activating Transcription Factor 1), both which bear high bZIP region sequence homology , and can bind as homo- or hetero-dimers with these members to the canonical cAMP Responsive Element (CRE) consensus sequence via the leucine zipper in the promoter regions of target genes [11, 22, 61]. The CREB/CREB homodimer exhibits a half-life of 10-20 minutes . Subsequent to phosphorylation at Ser133, CRE-bound CREB can exert its influence upon target gene transcription and interact with promoter-bound cofactors. The literature has so far less effectively studied the contribution of family members CREM and ATF, or their various isoforms.
An ever increasing body of evidence implicates the molecular actions of CREB in experimental paradigms related to addiction, a small number of which are demonstrated in Table 1. More recently, studies have examined the expression of pCREB following a variety of drugs of abuse employing in vivo models, off a background of over fifteen years of in vitro work with cell cultures. Largely, these studies have provided a conflicting array of results regarding positive and negative regulation of pCREB expression, with divergences particulary noted across brain regions examined and temporal follow-up, as well as the period of administration including acute, chronic and subsequent to precipitated withdrawal. Many of these details are demonstrated in Table 1, in particular the delay to neurochemical analysis as well as treatment paradigm.
Almost unfailingly, natural or precipitated withdrawal from drugs of abuse leads to a dramatic alteration in the expression of nuclear pCREB. Whilst the positive or negative nature of this impact appears dependent upon the brain region studied, substantial pCREB changes have been observed following withdrawal from ethanol (EtOH) [84, 86, 88], morphine [23, 41], psychostimulants (including: amphetamine , cocaine  and methamphetamine , MDMA ) and nicotine [15, 85, 94]. This may suggest a regional sensitization in the pCREB response, elsewhere deputised through CRE activation reporters [3, 7, 14, 105], providing a molecular stimulus for relapse behaviour. Together these data suggest that repeated exposure to multiple drugs of abuse may produce sustained activation of intracellular transcription factors, resulting in the persistent and altered expression of functionally important gene products that may underlie the onset and maintenance of addiction . Clearly, a role for pCREB in this process is beyond doubt, and it remains to us as investigators to fully unlock and characterise the methods and higher-level mechanics behind its action.
Of those papers listed in Table 1 and elsewhere  which examined the impact of drug upon nuclear CREB expression, few of them demonstrated some corresponding shift in its expression levels [74, 85, 94]. In contrast, substantial variation in the expression pattern of its activated form, pCREB, was observed. Such observations were made consistently across discrete brain regions and other physiologically significant organs, revealing little correlation between CREB and pCREB levels. It may be that CREB levels are more invariant to change given the necessary involvement gene transcription, translation and subsequent post-translational modification events, whereas, the changes in pCREB levels are merely indicative of protein kinase action.
A good reason for differential CREB or pCREB expression is that different central nuclei and cell populations offer various gene targets for these transcription factors, each likely exhibiting their own sensitivites to changes in the CREB transcription factor to induce gene transcription. Mayr and Montminy  tabulate some 105 genes with functional CRE motifs identified in the literature, half containing a single CGTA motif. One quarter of these CRE-bearing genes function in cellular metabolism, and the vast majority in some way could directly contribute to neural adaptation or synaptic plasticity following drug insult. Whilst a small number of CRE-bearing genes endogenous to the brain have been identifed in the literature, this observation underlies the likelihood that CREB is likely to impact differently in various neural regions. In a recent review, Carlezon and colleagues  highlight that CREB gene promoter targets number in their thousands and that not all CRE motif containing genes are functional CREB targets. In their words, ?the consequences of a change in CREB function, or in an upstream pathway, in various brain regions are [thus] likely to be multifaceted and difficult to predict a priori.?
Within the NAcc, for example, genes positively identified for upregulation by CREB include proenkephalin (Penk1), prodynorphin (Pdyn), and c-Fos (Fos) [2, 17, 72, 118], however, evidence supports a much broader extent of influence [71, 72], be it directly or indirectly via IEG induction. Psychostimulants have been shown to induce preprodynorphin mRNA in the NAcc through a CREB-mediated mechanism, and subsequently, these data have been used to establish an increasing recognition for dynorphin in its contribution to the expression of sensitization . Different CREB gene targets are identifed in differing central nuclei, although investitagor bias means that often gene targets are not consistently examined across nuclei. For example, Olson and colleagues  only examined GluR1 and TH genes as they are both known to contribute to drug reward in the VTA, with similar practises concerning opioids in the NAcc [17, 93, 108].
The collective in situ and immunoblotting data indicated that CREB and/or activated CREB are regulated by various drugs of abuse within brain regions implicated in addiction. These data raised the question as to the precise role for CREB in mediating addiction-related behaviours To this end, novel transgenic techniques were employed to knockout CREB and observe functional implications. The most obvious beginning was deletion of the DNA binding domain and all of the leucine zipper on the Creb gene, producing CREB-null mice (Crebnull) devoid of central and peripheral functional isoforms of CREB through inability to dimerise or bind to DNA (at the CRE site). Null mice were observed to have a reduced birth weight (70%) relative to wild type mice, were cyanotic, and died immediately after birth (perinatally) from respiratory distress (pulmonary atelectasis) . The mutant also exhibited other developmental abnormalities and phenotype including a birth rate less than Mendelian frequency, hypoplasia/atrophy of the corpus callosum and anterior commissure, a markedly reduced thymic cellularity affecting all developmental stages of the ??-T cell lineage, and upregulated CREM in the hippocampus and other forebrain regions; in contrast, wild type expression of CREM is predominantly restricted to neuroendocrine neurons . The total deletion of all central and peripheral isoforms of CREB were, despite the upregulation in CREM (which possibly allowed the mutant to reach parturition), the likely cause for these apparently diverse defects. Given the contribution of CREB to neuronal growth, plasticity and survival mediated by the neurotrophins including NGF, BDNF and NT-3 [63, 99, 114, 121], the impact of total CREB deletion was ubiquitious, substantially impairing normal organ and physiological development. It has been recently shown that mice with a null mutation for Creb restricted to central regions do not show the excessive apoptosis witnessed by peripheral Creb null mutation , driving subsequent studies into central-specific CREB recombination, discussed later.
An early attempt to create a CREB-null mutant led to the development of the now widely recognised Creb1?? mutant. Hummler and colleagues  created mutant mice by embryonic stem cell homologous recombination, inserting a promoterless Neo construct into the second exon of the Creb1 gene, which harbours the first ATG codon . They observed that the proportion of mutant mice surviving was down on expected Mendelian outcome, although adult mutants demonstrated a normal phenotype (measuring for ataxia/motor disorders/nociception/shock response) with no histological or morphological deficits . Blendy and colleagues  mused that the healthy disposition of mutants was puzzling because CRE-mediated gluconeogenic enzyme expression (critical for perinatal surivival) should be attenuated by the mutation, and that based on previous evidence of CREB in the pituitary, growth should be retarded. Indeed, while ATF-1 levels were unaffected in the mutants, RNA isoforms of CREM were upregulated (including the activator (CREM?) and repressor (CREM?, CREM?) isoforms, which lack an activator-specific exon), and a novel form of CREB, CREB-?, was observed to be upregulated . CREB-? carried a molecular weight of 40kDa and was identical to CREB-? (the prevalent CREB isoform) except for a deletion of 40 residues of the Q-domain; its upregulation was 6-fold in the brain and 4-fold in the liver, with upregulation also in the testes . CREM isoforms, previously shown to be expressed in (or constrained to) neuroendocrine nuclei, now became expressed ubiquitously throughout the brain . Such mutants have been described as carrying a hypomorphic CREB allele or as being haplodeficient in CREB , and a great deal of experimental work has been conducted using this model.
The balance of studies examining the impact of CREB knockdown in the context of addiction have done so with the CREB?? paradigm, probably because the model does not require time-consuming preparation and development, whilst still providing an interesting experimental subject. Such mutants do express higher than normal CREB-? levels, although studies have suggested [47, 66] and recently shown that total CREB activity is reduced approximately 90% throughout the brain (cortex, cerebellum, sub-cortical nuclei) . Clearly, the peripheral expression of CREB-?, though impaired, is enough to propagate required developmental homeostatic mechanisms in these mutants. The studies shown in Table 2 demonstrate a complex phenotype of this CREB knockout model. Numerous studies provide evidence that mutants have attenuated behavioral response to morphine withdrawal [66, 112, 115]. These mutants are basally more anxious than WT , which was demonstrated to impact upon EtOH (drug) preference rather than natural reward preference. Interestingly, whilst some studies demonstrate that cocaine and morphine earn a similar salience as well as exerting similar behavioural influence in mutants as wild types [59, 66, 112] others provide evidence to the contrary, or at least which hinders us in drawing any compelling conclusions [115, 117]. Moreover, Walters and colleagues  recently demonstrated that although 1mg/kg nicotine was rewarding to WT but not mutants, 2mg/kg nicotine elicited an aversive response in both mutants and WT?s, underlying the difficulty and complexity associated with interpretation of these results.
Collectively, these data emphasise the differential and unpredictable effect of diminished CREB throughout the CNS on behaviour and gene transcription [66, 87, 118], including various memory and learning conditions (for example, deficits observed in some but not all long term memory tests) [13, 34, 39]. No doubt complicating such results is the upregulation of CREM throughout the CNS so that it no longer distributes primarily in neuroendocrine-associated nuclei, as well as the upregulation in CREB-? , affecting the consistent reporting of affective and cognitive deficits. Whist this model is a step toward ascertaining the contribution of CREB to addiction, it is inferior to recent spatiotemporal models where CREB deletion is total, and the subject?s system has little time or scope to compensate for the knockout. Given the problems encountered with the peripheral knockdown of CREB, various novel models have been employed to target central expression of CREB, and where possible, to selectively target brain nuclei.
One such model is herpes simplex virus (HSV)-mediated overexpression of CREB in specific brain nuclei or, in order to gain a local knockdown of CREB, the dominant-negative isoform mCREB (mutant CREB). Mutant CREB (mCREB) contains a serine-to-alanine substitution at position 133, eliminating the cAMP-dependent protein kinase phosphorylation site but maintaining charge balance. Subsequently, whilst mCREB can still bind to cAMP responsive elements (CREs), it inhibits active CREB by occupying the CRE and preventing access by wild-type CREB and other CRE-binding proteins [98, 106]. The HSV-(m)CREB system achieves maximal expression of virally-encoded transgenes by 24 hours post-injection, which persists for three to four days before dissipating to trace or zero expression by day seven post-injection . A number of recent studies have examined the impact of HSV overexpression of (m)CREB in discrete regions of the CNS upon phenotype, shown in Table 3. The balance of these studies have as a key endpoint the impact of (m)CREB overexpression upon the rewarding effects of cocaine or morphine [7, 17, 93], finding that CREB overexpression in the NAcc shell decreases drug reward whilst mCREB enhances drug reward, results that may also apply to natural rewards . Such data suggest that immediately following drug intake, upregulation in accumbal CREB (or increased phosphorylation of CREB) may diminish the salience of further drug administration. This suggests a contribution to either early development of tolerance, or development of an inbuilt safety-mechanism which is activated as the body recovers from drug insult. Other findings using this system drive home the observation that regional CREB or mCREB overexpression has a markedly different impact upon either reward [17, 83] and affective states measured through phenotypic traits of anxiety or depression [7, 24, 93], or of physical dependence . Using recombinant Sindbis pseudovirions to drive constitutively active or dominant negative CREB overexpression in the NAcc, Dong and colleagues  demonstrated a recovery or further decrement in MSN excitability, respectively, in a rat model of cocaine bingeing.
This model is conceptually appealling, and indeed, the overexpression of dominant negative (mCREB) allows investigators to deduce the impact of substantial CREB knockdown in specific brain nuclei. In contrast, numerous studies have suggested that alterations in CREB levels per se following cross-temporal drug abuse seem to occur unpredictably, if at all. A far more consistent expression marker is its phosphorylated or active from, pCREB, and the cogent point made by Walters and colleageus  applies, ?Given that phosphorylated CREB (pCREB) is the transcriptionally active form of CREB and that a given stimulus might lead to the phosphorylation of only a few dozen molecules of CREB per cell, alterations in total CREB levels achieved by genetic manipulations might or might not lead to significant changes in pCREB depending on the original protein levels present?. In the balance of the HSV-CREB overexpression studies, experiments are conducted 2-3 days post-injection. Given that CREB (or more accurately, pCREB) expression shows a distinct and no doubt critically relevant temporal regulation following drug abuse stimuli, we are hard pressed to guess at what sort of remodelling effects prolonged CREB exposure may implement in discrete neural regions. Moreover, this overexpression period is unlikely to complement anything wrought upon CREB or pCREB expression by pharmacological or stressor methodology. Drugs of abuse alter pCREB levels directly through kinase pathways, and CREB levels indirectly through gene expression systems; however, viral (HSV) overexpression systems increase CREB directly, without altering pCREB levels directly through endogenously occuring systems. Subsequently, these results are somewhat difficult to interpret in regards of existing literature examining drugs of abuse in wild type mice given a differential method and time course in generating synaptic plasticity and neuroadapatation. Finally, whilst CREB is constitutively expressed in the nucleus, viral overexpression of CREB enters via the cytosol, suggestive of stochastic or unknown levels of trafficking into the nucleus to affect target CRE-binding and subsequent plasticity. Although the extent and kinetics of mCREB dimerisation (with CREB, CREM and ATF-1), CRE-DNA binding and subsequent dimer transcriptional activation potency is not fully known , HSV-mCREB overexpression appears to be a promising strategy in the ongoing elucidation of central CREB?s impact in the context of addiction studies.
Another attempt at regulating CREB expression in brain is the tetracycline transactivator system, whereby doxycycline in the drinking water is the ?switch? that is coupled to the suppression of transgene expression. CREB overexpression can subsequently be targeted to the brain, with a tetracycline transactivator (tTA) controlled by a 1.8kb neuron-specific enolase (NSE) promoter. Sakai and colleagues  crossed mouse lines to generate NSE-tTA TetOP-CREB? bi-transgenic mice, creating a system controlled by NSE, whose expression generates tTA, binding to the TetOP promoter, generating CREB? overexpression. Addition of a tetracycline analogue, doxycycline, binds to a Tet binding pocket on the tTA, which undergoes a conformational change and binds to TetOP in such a way, inhibiting CREB? expression. Their model demonstrated that CREB overexpression in the brain had an inconsistent influence upon expression of other members of the CREB/ATF family (invariably downregulating though), paricularly CREM. CREB overexpression was restricted to the nucleus of cells, predominantly in the striatum, although with some expression observed within the cingulate cortex and hippocampus. In contrast, Pittenger and colleagues used this model to overexpress dominant negative human CREB (KCREB) in either the dorsal striatum (and olfactory tubercle)  or dorsal hippocampus (CA1, and striatum/piriform cortex) , the former demonstrating a contribution of CREB to procedural learning, the latter, a somewhat subtle phenotype in relation to spatial learning and memory. A major downfall of this system, however, is the time lapse (seven to fourteen days) required to drive corresponding alteration in gene expression, a critical feature regarding (m/K)CREB cytological (dys)regulation.
Antisense models have also been utilised, involving the local knockdown of CREB by infusion of CREB antisense, which binds to Creb mRNA thus inhibiting further translation. A small number of studies (Table 4) employing CREB antisense collectively demonstrate the contribution of CREB to drug-induced behavioural phenotype and mRNA or protein expression. Whilst antisense seems to provide the ?magic bullet? to functional gene analysis, difficulty with delivery systems, specificity, toxicity and inconsistent effects are key drivers for its limited adoption in experimentation.
Underlying the aforementioned ?conventional? models of CREB manipulation is that while they have allowed insight, each has a major deficit which may confound interpretation. More recently, through various advances in the field of molecular biology, novel techniques have arisen which confer alternative targeting of the Creb gene and control of expression.
A more recent attempt to selectively target and knockdown CREB from central regions involves a model of region-specific promoters driving the site-specific Cre recombinase. This entails the cross-breeding of two strains of mice, one expressing the Cre transgene driven by a tissue- or ontogenetic stage-specific promoter, and the other a ?floxed? gene (loxP sequences flanking gene of interest for recombination) . Early problems with the model included the time taken to breed the strains, difficulty establishing well-characterised tissue-specific promoters, and the inability to induce Cre expression at specific ontogenetic time points . Over time, however, the latter two have been substantially ameliorated. As the expression of Cre-recombinase determines the recombination event surrounding the floxed gene, numerous approaches have been taken in addressing its expression profile. Various region-speicific Cre promoter models have been developed, including an adipose-specific aP2 enhancer/promoter , central- and ontogenetic-specific promoters CamKII? [18, 19, 51], Emx-1 [21, 38, 49], Foxg1 , Nestin [5, 68], a split-polypeptide Cre construct , tyrosine hydroxylase (TH) neuronal-specific promoter , RSV and EF1? promoters , rat insulin promoter [95, 96] and CAG promoter . The main design behind such mutants is to induce disruption of all central CREB isoforms through targeting of the 10thCreb1 exon. Excision of exon 10 from Creb1 leads to translation of CREB deficient in DNA binding and dimerization functional domains, such that the protein is unstable and thus, there is loss of CREB . The prominent applications of this technology to cellular CREB expression involves tissue-specific promoters, both regional and restricted by cell-type, as well as expression of promoters tied to developmental stages. Example applications of this model are discussed.
The nestin promoter/enhancer is associated with widespread central deletion of the Creb gene, as nestin expresses from embryonic stage in all brain regions before separation of neuronal and glial lineages , with CREB loss observed in neurons and glial cells. Creb1NesCre mice nominally reach 70-80% of control mouse body weight from 2nd post-natal week due to a deficiency in growth hormone. Data from nestin Creb-deficient mutants demonstrated no difference in CPP reward to morphine, cocaine or food versus wild type, but that they exhibited attenuated behavioural responses to naloxone-induced morphine withdrawal and had a heightened anxiogenic phenotype . CaMKII? is an 8.5kb promoter expressed in forebrain neurons from approximately P7 [5, 68] and this model has shown CREB loss in almost eighty percent of forebrain neurons. Indeed in transgenic mice, high CamKII? Cre levels have been demonstrated within the hippocampus, cortex, olfactory bulb and amygdala, and low levels in the striatum, thalamus and hypothalamus. No CamKII? was detected in the cerebellum . In these mice, CREB protein began to decrease in the hippocampus and cortex by P6 and was observed to be totally deficient in these regions by P15. Together, these mice models provide a robust method for knockdown of all functional CREB isoforms widely throughout the brain at different time points, without complex developmental adaptations.
CREM deficient mutants were generated to address the question of what role CREM plays in the absence of CREB, given the observation that CREM may upregulate subsequent to CREB knockout. Mantamadiotis and colleagues  studied transgenic mice with nestin and CaMKII? promoter-driven knockout of Creb1 crossed with Crem-deficient mice, generating pre-natal and post-natal CNS-specific protein loss respectively. Mice with prenatal central loss of CREB and CREM died at P1, as they did not suckle for milk; brain structures in these mice were unaffected, but cell density was markedly diminished, largely due to apoptosis; expected Mendelian ratios were observed however. Mice with postnatal central loss of CREB and CREM displayed an abnormal phenotype of retraction of limbs when suspended for 20s, characterising a neurological impairment owing to neurodegeneration, and considerable progressive atrophy of the dorsolateral striatum and CA1 hippocampal neurons; widespread astrogliosis was revealed (glial cell apoptosis) in dorsolateral striatum, CA1, DG and some in amygdala, cortex and thalamus, although a single copy of Crem prevented this (suggesting accomodation for loss of CREB); expected Mendelian ratios were observed. Furthermore, Crem-/- male mutant mice are known to be sterile, given enhanced apoptosis of post-meioitic germ cells , although the mutation does not appear to be as physiologically devastating as does total CREB deletion. CREM null mice have been previously described , exhibiting hyperactive and diminished anxiety-like behaviour, however, an addiction-related phenotype remains to be characterised for this model.
Other attempts at promoter-driven CREB deletion have aimed to target brain nuclei selectively, regions critical to the study of addiction. A D1A receptor gene promoter (Drd1a) was employed via 140kb YAC expression vector for Cre recombinase, generating a Cre expression profile predominantly constrained to the CPu, NAcc and OT, but with some expression observed in layer VI of the cortex, CA2 of the hippocampus and within thalamic nuclei. Also noted was neurodegeneration within the dorsolateral striatum when mutants were double crossed with CREM-deficient mice ; further phenotypic analysis is warranted in this model.
Dopamine and cAMP-regulated phosphoprotein molecular weight 32 kDA (DARPP-32) bears multiple regulatory phosphorylation sites, and acts as an important component in the phosphorylation profile of various cytosolic and membrane-associated proteins. Indeed, in its activated state as pThr34-DARPP-32, it becomes a robust inhibitor of protein phosphatase 1 (PP-1), a substrate responsible for de-phosphorylation of numerous cellular targets. DARPP-32 is highly concentrated in medium spiny neurons of the CPu and NAcc (neostriatal neurons), the olfactory tubercule, and lightly in the BNST and amygdala [40, 78]. A novel model of DARPP-32 promoter-driven Cre  will provide a conduit for the selective knockdown of CREB, allowing the elucidation of the contribution of CREB within the mesolimbic system; demonstrated in Fig. (2). Importantly, the expression of DARPP32 driven Cre in this model does not commence until 4-5 weeks after birth , ensuring a lack of potential developmental compensation.
A final model worth mentioning involves Emx1 (empty spiracles homologue 1), a homeobox-containing gene which is specifically expressed in the developing (from E10) telencephalic cortex through adulthood . Emx1 is involved in encoding transcription factors, and found in both proliferating and post-mitotic neurons of the cerebral cortex, and may thus be involved in the initiation and maintenance of the neuronal phenotype . Evidence supports its restriction to excitatory pyramidal neurons (layers II-VI, but not layer I) colocalising with glutamate (99.1%) rather than GABA (0.9%), as well as astrocytes and oligodentrocytes of most pallidal structures (vertebrate cerebral cortex primordium) including the hippocampus, neocortex, piriform cortex, endopiriform nucleus and lateral aspects of the amygdala [21, 38, 49]. Within the neocortex and hippocampus, approximately 88% of cells underwent recombination in total . This model is important, as it allows the study of CREB?s contribution to corticostriatal and corticotegmental glutamatergic pathways, both of which have both been strongly implicated in the development of addiction related behaviours, and is demonstrated in Fig. (2).
Whilst the promoter-driven strategy for central CREB deletion is good, some of these approaches are still germline in nature, thus suggestive that various difficult-to-identify compensatory adaptations take place in the organism, offsetting the accuracy of experimental results. Correspondingly, investigators have also turned to plasmid based transgene expression systems, however, these tend to be influenced by chromatin position effects and may result in ectopic or mosaic expression. Such plasmid-based transgenes, containing only a few regulatory elements from the gene of interest, often result in unnatural configurations and generally produce highly variable transgene expression patterns . Consequently, many transgenic lines need to be analysed to find one line with the appropriate pattern of expression. A more appropriate and attractive approach used by investigators is that of viral vector based Cre delivery systems. This strategy is useful when the phenotype of the total knockout is lethal (embryological mutation, as is the case with CREB), when there are compensatory effects during development (in regard to other gene expression), or when total gene inactivation results in pleiotropic effects involving complex physiological interactions. Viral-vector delivered Cre recombinase provides a spatiotemporal model for knocking out ?floxed? genes in specific nuclei through stereotaxic injection targeting a brain region or cell group. This novel approach to generating conditional knockouts has been validated on numerous occasions, many involving Rosa 26 Reporter (Rosa26) mice or ?-galactosidase expression systems [1, 4, 50, 89, 119].
Two widely used virus strains for this purpose are the lentivirus and adeno-associated virus. Lentiviruses (LV) are enveloped RNA viruses that belong to the family of complex retroviruses. In contrast to prototypic retroviruses, such as murine leukemia virus, lentiviruses are able to transduce both dividing and nondividing cells, including neuronal and to a lesser extent glial (astrocyte) recombination. Adeno-associated viral (AAV) vectors induce a substantially lower immunogenic dose than its predecessors, given the total removal of viral genome. AAV is selective toward neuronal recombination in vivo, with evidence that it does not recombine in glial cells . Optimal expression is usually 2-4 weeks following injection. Given that AAV and LV are useful in mediating gene delivery and stable transduction to both dividing and non-dividing cells, whilst not inducing an immune response, they provide a valuable role in CNS recombination. AAV vectors are typically limited to accepting only short inserts of up to 5kb , however a high capacity adenoviral vector expressing Cre recombinase has been described . In this case, all viral coding sequences have been deleted from the viral genome, reducing the expression vector?s toxicity and immunogenicity, whilst increasing the capacity for introduction of heterologous DNA to 36kB, facilitating increased flexibility. Viral-delivered Cre can be toxic, likely owing to mammalian pseudo-loxP sequence homology [1, 89], where heightened Cre can reduce proliferation , induce chromosomal abberation and exchanges with sister chromatids , and cause neural (brain) cavities. Chronic high-level expression of Cre in spermatids of transgenic mice has also been demonstrated to generate male sterility . This has been addressed by using lower Cre doses, whilst still achieving substantial recombination , or use of self-deleting viral vectors. The latter has been pioneered with lentiviral delivery of Cre, such that the U3 untranslated region (3? LTR) of the LV vector has a Cre insert which expresses only once incorporated into the mammalian genome, self-removing from the genome as Cre concentration rises [89, 107]. In the context of the study of addiction, application of this model to selectively target brain regions critical to the development of addiction will prove valuable in determining the contribution of CREB to various pathways of the midbrain and forebrain.
These spatiotemporal paradigms are important indeed, critical, to more efficacious targeting of CREB knockdown in specific cells as well as nuclei. This can better assist in diagnosis of the diverse role CREB plays in neuronal signalling, contributing to addictive behaviours and / or how this phenotype relates to molecular signalling pathways, when both are used in unison. As these models are more widely adopted over subsequent years, the data on the contribution of CREB to the development and expression of addiction should be more reliable, and help us better understand its biological function.
This review has briefly discussed the relevance of CREB as a critical biological substrate for the development of addiction, and examined a variety of drug contexts and paradigms with wild type subjects demonstrating its diverse and complex expression pattern. Earlier models, which endeavoured to remove peripheral or central CREB isoforms to determine its impact upon addiction more closely, were found to be in various ways deficient and inadequate for robust and consistent reporting. More recently, spatiotemporal CREB deletion models have provided an illuminating and exciting way forward for the study of total central CREB deletion both temporally, and spatially within the brain, allowing investigators to better characterise precisely the contribution of CREB to the development and / or persistence of the addicted state.
The authors would like to acknowledge ongoing funding from the NHMRC and ARC.
|ATF-1||= Activating transcription factor-1|
|CaMK||= Ca2+/calmodulin protein kinase|
|CREB||= cAMP responsive element binding protein|
|CREM||= cAMP responsive element modulatory protein|
|LC||= Locus coeruleus|
|NAcc||= Nucleus accumbens|
|PVN||= paraventricular nucleus of the hypothalamus;|
|1.||Ahmed BY,Chakravarthy S,Eggers R,Hermens WT,Zhang JY,Niclou SP,Levelt C,Sablitzky F,Anderson PN,Lieberman AR,Verhaagen J. Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectorsBMC Neurosci 2004;5:4. [pmid: 15005815]|
|2.||Andersson M,Konradi C,Cenci MA. cAMP response element-binding protein is required for dopamine-dependent gene expression in the intact but not the dopamine-denervated striatumJ. Neurosci 2001;21:9930–9943. [pmid: 11739600]|
|3.||Asyyed A,Storm D,Diamond I. Ethanol activates cAMP response element-mediated gene expression in select regions of the mouse brainBrain Res 2006;1106:63–71. [pmid: 16854384]|
|4.||Badorf M,Edenhofer F,Dries V,Kochanek S,Schiedner G. Efficient in vitro and In vivo excision of floxed sequences with a high-capacity adenoviral vector expressing Cre recombinaseGenesis 2002;33:119–124. [pmid: 12124944]|
|5.||Balschun D,Wolfer DP,Gass P,Mantamadiotis T,Welzl H,Schutz G,Frey JU,Lipp HP. Does cAMP response element-binding protein have a pivotal role in hippocampal synaptic plasticity and hippocampus-dependent memory?J. Neurosci 2003;23:6304–6314. [pmid: 12867515]|
|6.||Barlow C,Schroeder M,Lekstrom-Himes J,Kylefjord H,Deng CX,Wynshaw-Boris A,Spiegelman BM,Xanthopoulos KG. Targeted expression of Cre recombinase to adipose tissue of transgenic mice directs adipose-specific excision of loxP-flanked gene segmentsNucleic Acids Res 1997;25:2543–2545. [pmid: 9171115]|
|7.||Barrot M,Olivier JD,Perrotti LI,DiLeone RJ,Berton O,Eisch AJ,Impey S,Storm DR,Neve RL,Yin JC,Zachariou V,Nestler EJ. CREB activity in the nucleus accumbens shell controls gating of behavioral responses to emotional stimuliProc. Natl. Acad. Sci. USA 2002;99:11435–11440. [pmid: 12165570]|
|8.||Berke JD,Hyman SE. Addiction, dopamine, and the molecular mechanisms of memoryNeuron 2000;25:515–532. [pmid: 10774721]|
|9.||Berridge KC,Robinson TE. The mind of an addicted brain neural sensitization of wanting versus likingCurr. Dir. Psychol. Sci 1995;4:71–75.|
|10.||Blendy JA,Kaestner KH,Schmid W,Gass P,Schutz G. Targeting of the CREB gene leads to up-regulation of a novel CREB mRNA isoformEMBO J 1996;15:1098–1106. [pmid: 8605879]|
|11.||Blendy JA,Maldonado R. Genetic analysis of drug addiction: the role of cAMP response element binding proteinJ. Mol. Med 1998;76:104–110. [pmid: 9500675]|
|12.||Bogush AI,McCarthy LE,Tian C,Olm V,Gieringer T,Ivkovic S,Ehrlich ME. DARPP-32 genomic fragments drive Cre expression in postnatal striatumGenesis 2005;42:37–46. [pmid: 15830379]|
|13.||Bourtchuladze R,Frenguelli B,Blendy J,Cioffi D,Schutz G,Silva AJ. Deficient long-term memory in mice with a targeted mutation of the cAMP-responsive element-binding proteinCell 1994;79:59–68. [pmid: 7923378]|
|14.||Brodie CR,Khaliq M,Yin JC,Brent Clark H,Orr HT,Boland LM. Overexpression of CREB reduces CRE-mediated transcription: behavioral and cellular analyses in transgenic miceMol. Cell Neurosci 2004;25:602–611. [pmid: 15080890]|
|15.||Brunzell DH,Russell DS,Picciotto MR. In vivo nicotine treatment regulates mesocorticolimbic CREB and ERK signaling in C57Bl/6J miceJ. Neurochem 2003;84:1431–1441. [pmid: 12614343]|
|16.||Carlezon WA, Jr;Duman, RS.;Nestler, EJ.The many faces of CREBTrends Neurosci 2005;28:436–445. [pmid: 15982754]|
|17.||Carlezon WA, Jr;Thome, J.;Olson, VG.;Lane-Ladd, SB.;Brodkin, ES.;Hiroi, N.;Duman, RS.;Neve, RL.;Nestler, EJ.Regulation of cocaine reward by CREBScience 1998;282:2272–2275. [pmid: 9856954]|
|18.||Casanova E,Fehsenfeld S,Lemberger T,Shimshek DR,Sprengel R,Mantamadiotis T. ER-based double iCre fusion protein allows partial recombination in forebrainGenesis 2002;34:208–214. [pmid: 12395386]|
|19.||Casanova E,Fehsenfeld S,Mantamadiotis T,Lemberger T,Greiner E,Stewart AF,Schutz G. A CamKIIalpha iCre BAC allows brain-specific gene inactivationGenesis 2001;31:37–42. [pmid: 11668676]|
|20.||Casanova E,Lemberger T,Fehsenfeld S,Mantamadiotis T,Schutz G. Alpha complementation in the Cre recombinase enzymeGenesis 2003;37:25–29. [pmid: 14502574]|
|21.||Chan CH,Godinho LN,Thomaidou D,Tan SS,Gulisano M,Parnavelas JG. Emx1 is a marker for pyramidal neurons of the cerebral cortexCereb. Cortex 2001;11:1191–1198. [pmid: 11709490]|
|22.||Chao J,Nestler EJ. Molecular neurobiology of drug addictionAnnu. Rev. Med 2004;55:113–132. [pmid: 14746512]|
|23.||Chartoff EH,Papadopoulou M,Konradi C,Carlezon WA Jr. Dopamine-dependent increases in phosphorylation of cAMP response element binding protein (CREB) during precipitated morphine withdrawal in primary cultures of rat striatumJ. Neurochem 2003;87:107–118. [pmid: 12969258]|
|24.||Chen AC,Shirayama Y,Shin KH,Neve RL,Duman RS. Expression of the cAMP response element binding protein (CREB) in hippocampus produces an antidepressant effectBiol. Psychiatry 2001;49:753–762. [pmid: 11331083]|
|25.||Choi KH,Whisler K,Graham DL,Self DW. Antisense-induced reduction in nucleus accumbens cyclic AMP response element binding protein attenuates cocaine reinforcementNeuroscience 2006;137:373–383. [pmid: 16359811]|
|26.||Cole RL,Konradi C,Douglass J,Hyman SE. Neuronal adaptation to amphetamine and dopamine: molecular mechanisms of prodynorphin gene regulation in rat striatumNeuron 1995;14:813–823. [pmid: 7718243]|
|27.||Dalley JW,Thomas KL,Howes SR,Tsai TH,Aparicio-Legarza MI,Reynolds GP,Everitt BJ,Robbins TW. Effects of excitotoxic lesions of the rat prefrontal cortex on CREB regulation and presynaptic markers of dopamine and amino acid function in the nucleus accumbensEur. J. Neurosci 1999;11:1265–1274. [pmid: 10103121]|
|28.||Das S,Grunert M,Williams L,Vincent SR. NMDA and D1 receptors regulate the phosphorylation of CREB and the induction of c-fos in striatal neurons in primary cultureSynapse 1997;25:227–233. [pmid: 9068120]|
|29.||Dong Y,Green T,Saal D,Marie H,Neve R,Nestler EJ,Malenka RC. CREB modulates excitability of nucleus accumbens neuronsNat. Neurosci 2006;9:475–477. [pmid: 16520736]|
|30.||Everitt BJ,Morris KA,O'Brien A,Robbins TW. The basolateral amygdala-ventral striatal system and conditioned place preference: further evidence of limbic-striatal interactions underlying reward-related processesNeuroscience 1991;42:1–18. [pmid: 1830641]|
|31.||Fuchs RA,Branham RK,See RE. Different neural substrates mediate cocaine seeking after abstinence versus extinction training: a critical role for the dorsolateral caudate-putamenJ. Neurosci 2006;26:3584–3588. [pmid: 16571766]|
|32.||Gagneten S,Le Y,Miller J,Sauer B. Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletionsNucleic Acids Res 1997;25:3326–3331. [pmid: 9241248]|
|33.||Gao C,Che LW,Chen J,Xu XJ,Chi ZQ. Ohmefentanyl stereoisomers induce changes of CREB phosphorylation in hippocampus of mice in conditioned place preference paradigmCell Res 2003;13:29–34. [pmid: 12643347]|
|34.||Gass P,Wolfer DP,Balschun D,Rudolph D,Frey U,Lipp HP,Schutz G. Deficits in memory tasks of mice with CREB mutations depend on gene dosageLearn Mem 1998;5:274–288. [pmid: 10454354]|
|35.||Gelman DM,Noain D,Avale ME,Otero V,Low MJ,Rubinstein M. Transgenic mice engineered to target Cre/loxP-mediated DNA recombination into catecholaminergic neuronsGenesis 2003;36:196–202. [pmid: 12929090]|
|36.||Genova LM,Hyman SE. 5-HT3 receptor activation is required for induction of striatal c-Fos and phosphorylation of ATF-1 by amphetamineSynapse 1998;30:71–78. [pmid: 9704883]|
|37.||Gold LH,Swerdlow NR,Koob GF. The role of mesolimbic dopamine in conditioned locomotion produced by amphetamineBehav. Neurosci 1988;102:544–552. [pmid: 3139012]|
|38.||Gorski JA,Talley T,Qiu M,Puelles L,Rubenstein JL,Jones KR. Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineageJ. Neurosci 2002;22:6309–6314. [pmid: 12151506]|
|39.||Graves L,Dalvi A,Lucki I,Blendy JA,Abel T. Behavioral analysis of CREB alphadelta mutation on a B6/129 F1 hybrid backgroundHippocampus 2002;12:18–26. [pmid: 11918283]|
|40.||Greengard P. The neurobiology of slow synaptic transmissionScience 2001;294:1024–1030. [pmid: 11691979]|
|41.||Guitart X,Thompson MA,Mirante CK,Greenberg ME,Nestler EJ. Regulation of cyclic AMP response element-binding protein (CREB) phosphorylation by acute and chronic morphine in the rat locus coeruleusJ. Neurochem 1992;58:1168–1171. [pmid: 1531356]|
|42.||Han M,Bolanos CA,Green TA,Olson VG,Neve RL,Liu R,Aghajanian GK,Nestler EJ. Role of cAMP response element-binding protein in the rat locus ceruleus: regulation of neuronal activity and opiate withdrawal behaviorsJ. Neurosci 2006;26:4624–4629. [pmid: 16641242]|
|43.||Hebert JM,McConnell SK. Targeting of cre to the Foxg1 (BF-1) locus mediates loxP recombination in the telencephalon and other developing head structuresDev. Biol 2000;222:296–306. [pmid: 10837119]|
|44.||Hiremagalur B,Sabban EL. Nicotine elicits changes in expression of adrenal catecholamine biosynthetic enzymes, neuropeptide Y and immediate early genes by injection but not continuous administrationBrain Res. Mol. Brain Res 1995;32:109–115. [pmid: 7494448]|
|45.||Hiroi N,White NM. The lateral nucleus of the amygdala mediates expression of the amphetamine-produced conditioned place preferenceJ. Neurosci 1991;11:2107–2116. [pmid: 2066777]|
|46.||Hsieh HC,Li HY,Lin MY,Chiou YF,Lin SY,Wong CH,Chen JC. Spatial and temporal profile of haloperidol-induced immediate-early gene expression and phosphoCREB binding in the dorsal and ventral striatum of amphetamine-sensitized ratsSynapse 2002;45:230–244. [pmid: 12125044]|
|47.||Hummler E,Cole TJ,Blendy JA,Ganss R,Aguzzi A,Schmid W,Beermann F,Schutz G. Targeted mutation of the CREB gene: compensation within the CREB/ATF family of transcription factorsProc Natl. Acad. Sci. USA 1994;91:5647–5651. [pmid: 8202542]|
|48.||Ito R,Robbins TW,Everitt BJ. Differential control over cocaine-seeking behavior by nucleus accumbens core and shellNat. Neurosci 2004;7:389–397. [pmid: 15034590]|
|49.||Iwasato T,Nomura R,Ando R,Ikeda T,Tanaka M,Itohara S. Dorsal telencephalon-specific expression of Cre recombinase in PAC transgenic miceGenesis 2004;38:130–138. [pmid: 15048810]|
|50.||Kaspar BK,Vissel B,Bengoechea T,Crone S,Randolph-Moore L,Muller R,Brandon EP,Schaffer D,Verma IM,Lee KF,Heinemann SF,Gage FH. Adeno-associated virus effectively mediates conditional gene modification in the brainProc. Natl. Acad. Sci. USA 2002;99:2320–2325. [pmid: 11842206]|
|51.||Kellendonk C,Tronche F,Casanova E,Anlag K,Opherk C,Schutz G. Inducible site-specific recombination in the brainJ. Mol. Biol 1999;285:175–182. [pmid: 9878397]|
|52.||Kelley AE. Memory and addiction: shared neural circuitry and molecular mechanismsNeuron 2004;44:161–179. [pmid: 15450168]|
|53.||Kelley AE,Berridge KC. The neuroscience of natural rewards: relevance to addictive drugsJ. Neurosci 2002;22:3306–3311. [pmid: 11978804]|
|54.||Kim YH,Won JS,Won MH,Lee JK,Suh HW. Role of proto-oncogenes in the regulation of proenkephalin mRNA expression induced by repeated nicotine injections in rat adrenal medullaLife Sci 2002;70:2915–2929. [pmid: 12269402]|
|55.||King GR,Xiong Z,Douglas S,Lee TH,Ellinwood EH. The effects of continuous cocaine dose on the induction of behavioral tolerance and dopamine autoreceptor functionEur. J. Pharmacol 1999;376:207–215. [pmid: 10448878]|
|56.||Konradi C,Cole RL,Heckers S,Hyman SE. Amphetamine regulates gene expression in rat striatum via transcription factor CREBJ. Neurosci 1994;14:5623–5634. [pmid: 8083758]|
|57.||Koob GF,Caine SB,Parsons L,Markou A,Weiss F. Opponent process model and psychostimulant addictionPharmacol. Biochem. Behav 1997;57:513–521. [pmid: 9218276]|
|58.||Koob GF,Sanna PP,Bloom FE. Neuroscience of addictionNeuron 1998;21:467–476. [pmid: 9768834]|
|59.||Kreibich AS,Blendy JA. cAMP response element-binding protein is required for stress but not cocaine-induced reinstatementJ. Neurosci 2004;24:6686–6692. [pmid: 15282271]|
|60.||Kuo Y,Liang K,Chen H,Cherng C,Lee H,Lin Y,Huang A,Liao R,Yu L. Cocaine-but not methamphetamine-associated memory requires de novo protein synthesisNeurobiol. Learn. Mem 2007;87:93–100. [pmid: 16905344]|
|61.||Lakatos A,Dominguez G,Kuhar MJ. CART promoter CRE site binds phosphorylated CREBBrain Res. Mol. Brain Res 2002;104:81–85. [pmid: 12117553]|
|62.||Lane-Ladd SB,Pineda J,Boundy VA,Pfeuffer T,Krupinski J,Aghajanian GK,Nestler EJ. CREB (cAMP response element-binding protein) in the locus coeruleus: biochemical, physiological, and behavioral evidence for a role in opiate dependenceJ. Neurosci 1997;17:7890–7901. [pmid: 9315909]|
|63.||Lonze BE,Riccio A,Cohen S,Ginty DD. Apoptosis axonal growth defects, and degeneration of peripheral neurons in mice lacking CREBNeuron 2002;34:371–385. [pmid: 11988169]|
|64.||Loonstra A,Vooijs M,Beverloo HB,Allak BA,van Drunen E,Kanaar R,Berns A,Jonkers J. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cellsProc. Natl. Acad. Sci. USA 2001;98:9209–9214. [pmid: 11481484]|
|65.||Loriaux MM,Rehfuss RP,Brennan RG,Goodman RH. Engineered leucine zippers show that hemiphosphorylated CREB complexes are transcriptionally activeProc. Natl. Acad. Sci. USA 1993;90:9046–9050. [pmid: 8105470]|
|66.||Maldonado R,Blendy JA,Tzavara E,Gass P,Roques BP,Hanoune J,Schutz G. Reduction of morphine abstinence in mice with a mutation in the gene encoding CREBScience 1996;273:657–659. [pmid: 8662559]|
|67.||Maldonado R,Smadja C,Mazzucchelli C,Sassone-Corsi P. Altered emotional and locomotor responses in mice deficient in the transcription factor CREMProc. Natl. Acad. Sci. USA 1999;96:14094–14099. [pmid: 10570204]|
|68.||Mantamadiotis T,Lemberger T,Bleckmann SC,Kern H,Kretz O,Martin Villalba A,Tronche F,Kellendonk C,Gau D,Kapfhammer J,Otto C,Schmid W,Schutz G. Disruption of CREB function in brain leads to neurodegenerationNat. Genet 2002;31:47–54. [pmid: 11967539]|
|69.||Mantamadiotis T,Sch?tz G,Maldonado R. Maldonado RMolecular Genetic ApproachesMolecular Biology of Drug Addiction 2002New Jersey: Humana Press; :360.|
|70.||Martinez-Turrillas R,Moyano S,Del Rio J,Frechilla D. Differential effects of 3,4-methylenedioxymethamphetamine (MDMA ?ecstasy?) on BDNF mRNA expression in rat frontal cortex and hippocampusNeurosci. Lett 2006;402:126–130. [pmid: 16644117]|
|71.||Mayr B,Montminy M. Transcriptional regulation by the phosphorylation-dependent factor CREBNat. Rev. Mol. Cell Biol 2001;2:599–609. [pmid: 11483993]|
|72.||McClung CA,Nestler EJ. Regulation of gene expression and cocaine reward by CREB and DeltaFosBNat. Neurosci 2003;6:1208–1215. [pmid: 14566342]|
|73.||McClung CA,Ulery PG,Perrotti LI,Zachariou V,Berton O,Nestler EJ. DeltaFosB: a molecular switch for long-term adaptation in the brainBrain Res. Mol. Brain Res 2004;132:146–154. [pmid: 15582154]|
|74.||McDaid J,Dallimore JE,Mackie AR,Celeste Napier T. Changes in accumbal and pallidal pCREB and deltaFosB in morphine-sensitized rats: correlations with receptor-evoked electrophysiological measures in the ventral pallidumNeuropsychopharmacology 2006;31:1212–1226. [pmid: 16123760]|
|75.||McDaid J,Graham MP,Napier TC. Methamphetamine-induced sensitization differentially alters pCREB and delta-FosB throughout the limbic circuit of the mammalian brainMol. Pharmacol 2006;70:2064–2074. [pmid: 16951039]|
|76.||Misra K,Roy A,Pandey SC. Effects of voluntary ethanol intake on the expression of Ca(2+) /calmodulin-dependent protein kinase IV and on CREB expression and phosphorylation in the rat nucleus accumbensNeuroreport 2001;12:4133–4137. [pmid: 11742252]|
|77.||Montminy MR,Bilezikjian LM. Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin geneNature 1987;328:175–178. [pmid: 2885756]|
|78.||Nairn AC,Svenningsson P,Nishi A,Fisone G,Girault JA,Greengard P. The role of DARPP-32 in the actions of drugs of abuseNeuropharmacology 2004;47(1):14–23. [pmid: 15464122]|
|79.||Nestler EJ. Molecular mechanisms of opiate and cocaine addictionCurr. Opin. Neurobiol 1997;7:713–719. [pmid: 9384550]|
|80.||Nestler EJ. Molecular neurobiology of addictionAm. J. Addict 2001;10:201–217. [pmid: 11579619]|
|81.||Nestler EJ. Common molecular and cellular substrates of addiction and memoryNeurobiol. Learn Mem 2002;78:637–647. [pmid: 12559841]|
|82.||Nestler EJ. Molecular mechanisms of drug addictionNeuropharmacology 2004;47(Suppl 1):24–32. [pmid: 15464123]|
|83.||Olson VG,Zabetian CP,Bolanos CA,Edwards S,Barrot M,Eisch AJ,Hughes T,Self DW,Neve R,Nestler E. Regulation of drug reward by cAMP response element-binding protein: evidence for two functionally distinct subregions of the ventral tegmental areaJ. Neurosci 2005;25:5553–5562. [pmid: 15944383]|
|84.||Pandey SC,Roy A,Mittal N. Effects of chronic ethanol intake and its withdrawal on the expression and phosphorylation of the creb gene transcription factor in rat cortexJ. Pharmacol. Exp. Ther 2001;296:857–868. [pmid: 11181917]|
|85.||Pandey SC,Roy A,Xu T,Mittal N. Effects of protracted nicotine exposure and withdrawal on the expression and phosphorylation of the CREB gene transcription factor in rat brainJ. Neurochem 2001;77:943–952. [pmid: 11331423]|
|86.||Pandey SC,Roy A,Zhang H. The decreased phosphorylation of cyclic adenosine monophosphate (cAMP) response element binding (CREB) protein in the central amygdala acts as a molecular substrate for anxiety related to ethanol withdrawal in ratsAlcohol. Clin. Exp. Res 2003;27:396–409. [pmid: 12658105]|
|87.||Pandey SC,Roy A,Zhang H,Xu T. Partial deletion of the cAMP response element-binding protein gene promotes alcohol-drinking behaviorsJ. Neurosci 2004;24:5022–5030. [pmid: 15163695]|
|88.||Pandey SC,Zhang D,Mittal N,Nayyar D. Potential role of the gene transcription factor cyclic AMP-responsive element binding protein in ethanol withdrawal-related anxietyJ. Pharmacol. Exp. Ther 1999;288:866–878. [pmid: 9918601]|
|89.||Pfeifer A,Brandon EP,Kootstra N,Gage FH,Verma IM. Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivoProc. Natl. Acad. Sci. USA 2001;98:11450–11455. [pmid: 11553794]|
|90.||Pierce RC,Kalivas PW. A circuitry model of the expression of behavioral sensitization to amphetamine-like psychostimulantsBrain Res. Brain Res. Rev 1997;25:192–216. [pmid: 9403138]|
|91.||Pittenger C,Fasano S,Mazzocchi-Jones D,Dunnett SB,Kandel ER,Brambilla R. Impaired bidirectional synaptic plasticity and procedural memory formation in striatum-specific cAMP response element-binding protein-deficient miceJ. Neurosci 2006;26:2808–2813. [pmid: 16525060]|
|92.||Pittenger C,Huang YY,Paletzki RF,Bourtchouladze R,Scanlin H,Vronskaya S,Kandel ER. Reversible inhibition of CREB/ATF transcription factors in region CA1 of the dorsal hippocampus disrupts hippocampus-dependent spatial memoryNeuron 2002;34:447–462. [pmid: 11988175]|
|93.||Pliakas AM,Carlson RR,Neve RL,Konradi C,Nestler EJ,Carlezon WA Jr. Altered responsiveness to cocaine and increased immobility in the forced swim test associated with elevated cAMP response element-binding protein expression in nucleus accumbensJ. Neurosci 2001;21:7397–7403. [pmid: 11549750]|
|94.||Pluzarev O,Pandey SC. Modulation of CREB expression and phosphorylation in the rat nucleus accumbens during nicotine exposure and withdrawalJ. Neurosci. Res 2004;77:884–891. [pmid: 15334606]|
|95.||Ray MK,Fagan SP,Moldovan S,DeMayo FJ,Brunicardi FC. A mouse model for beta cell-specific ablation of target gene(s) using the Cre-loxP systemBiochem. Biophys. Res. Commun 1998;253:65–69. [pmid: 9875221]|
|96.||Ray MK,Fagan SP,Moldovan S,DeMayo FJ,Brunicardi FC. Beta cell-specific ablation of target gene using Cre-loxP system in transgenic miceJ. Surg. Res 1999;84:199–203. [pmid: 10357920]|
|97.||Robinson TE,Berridge KC. The psychology and neurobiology of addiction: an incentive-sensitization viewAddiction 2000;95(2):S91–117. [pmid: 11002906]|
|98.||Rudolph D,Tafuri A,Gass P,Hammerling GJ,Arnold B,Schutz G. Impaired fetal T cell development and perinatal lethality in mice lacking the cAMP response element binding proteinProc. Natl. Acad. Sci. USA 1998;95:4481–4486. [pmid: 9539763]|
|99.||Saini HS,Gorse KM,Boxer LM,Sato-Bigbee C. Neurotrophin-3 and a CREB-mediated signaling pathway regulate Bcl-2 expression in oligodendrocyte progenitor cellsJ. Neurochem 2004;89:951–961. [pmid: 15140194]|
|100.||Sakai K,Miyazaki J. A transgenic mouse line that retains Cre recombinase activity in mature oocytes irrespective of the cre transgene transmissionBiochem. Biophys. Res. Commun 1997;237:318–324. [pmid: 9268708]|
|101.||Sakai N,Thome J,Newton SS,Chen J,Kelz MB,Steffen C,Nestler EJ,Duman RS. Inducible and brain region-specific CREB transgenic miceMol. Pharmacol 2002;61:1453–1464. [pmid: 12021407]|
|102.||Schmidt EE,Taylor DS,Prigge JR,Barnett S,Capecchi MR. Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatidsProc. Natl. Acad Sci. USA 2000;97:13702–13707. [pmid: 11087830]|
|103.||Self DW,Genova LM,Hope BT,Barnhart WJ,Spencer JJ,Nestler EJ. Involvement of cAMP-dependent protein kinase in the nucleus accumbens in cocaine self-administration and relapse of cocaine-seeking behaviorJ. Neurosci 1998;18:1848–1859. [pmid: 9465009]|
|104.||Self DW,Nestler EJ. Molecular mechanisms of drug reinforcement and addictionAnnu. Rev. Neurosci 1995;18:463–495. [pmid: 7605071]|
|105.||Shaw-Lutchman TZ,Barrot M,Wallace T,Gilden L,Zachariou V,Impey S,Duman RS,Storm D,Nestler EJ. Regional and cellular mapping of cAMP response element-mediated transcription during naltrexone-precipitated morphine withdrawalJ. Neurosci 2002;22:3663–3672. [pmid: 11978842]|
|106.||Shaywitz AJ,Greenberg ME. CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signalsAnnu. Rev. Biochem 1999;68:821–861. [pmid: 10872467]|
|107.||Silver DP,Livingston DM. Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicityMol. Cell 2001;8:233–243. [pmid: 11511376]|
|108.||Simpson JN,McGinty JF. Forskolin induces preproenkephalin and preprodynorphin mRNA in rat striatum as demonstrated by in situ hybridization histochemistrySynapse 1995;19:151–159. [pmid: 7784955]|
|109.||Solomon RL,Corbit JD. An opponent-process theory of motivation.I. Temporal dynamics of affectPsychol. Rev 1974;81:119–145. [pmid: 4817611]|
|110.||Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strainNat. Genet 1999;21:70–71. [pmid: 9916792]|
|111.||Turgeon SM,Pollack AE,Fink JS. Enhanced CREB phosphorylation and changes in c-Fos and FRA expression in striatum accompany amphetamine sensitizationBrain Res 1997;749:120–126. [pmid: 9070635]|
|112.||Valverde O,Mantamadiotis T,Torrecilla M,Ugedo L,Pineda J,Bleckmann S,Gass P,Kretz O,Mitchell JM,Schutz G,Maldonado R. Modulation of anxiety-like behavior and morphine dependence in CREB-deficient miceNeuropsychopharmacology 2004;29:1122–1133. [pmid: 15029152]|
|113.||Vanderschuren LJ,Di Ciano P,Everitt BJ. Involvement of the dorsal striatum in cue-controlled cocaine seekingJ. Neurosci 2005;25:8665–8670. [pmid: 16177034]|
|114.||Visvader J,Sassone-Corsi P,Verma IM. Two adjacent promoter elements mediate nerve growth factor activation of the c-fos gene and bind distinct nuclear complexesProc. Natl. Acad. Sci. USA 1988;85:9474–9478. [pmid: 2849107]|
|115.||Walters CL,Blendy JA. Different requirements for cAMP response element binding protein in positive and negative reinforcing properties of drugs of abuseJ. Neurosci 2001;21:9438–9444. [pmid: 11717377]|
|116.||Walters CL,Cleck JN,Kuo YC,Blendy JA. Mu-opioid receptor and CREB activation are required for nicotine rewardNeuron 2005;46:933–943. [pmid: 15953421]|
|117.||Walters CL,Godfrey M,Li X,Blendy JA. Alterations in morphine-induced reward, locomotor activity and thermoregulation in CREB-deficient miceBrain Res 2005;1032:193–199. [pmid: 15680959]|
|118.||Walters CL,Kuo YC,Blendy JA. Differential distribution of CREB in the mesolimbic dopamine reward pathwayJ. Neurochem 2003;87:1237–1244. [pmid: 14622103]|
|119.||Wang Y,Krushel LA,Edelman GM. Targeted DNA recombination In vivo using an adenovirus carrying the cre recombinase geneProc. Natl. Acad. Sci. USA 1996;93:3932–3936. [pmid: 8632992]|
|120.||Widnell KL,Self DW,Lane SB,Russell DS,Vaidya VA,Miserendino MJ,Rubin CS,Duman RS,Nestler EJ. Regulation of CREB expression: in vivo evidence for a functional role in morphine action in the nucleus accumbensJ. Pharmacol. Exp. Ther 1996;276:306–315. [pmid: 8558448]|
|121.||Xing J,Kornhauser JM,Xia Z,Thiele EA,Greenberg ME. Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylationMol. Cell Biol 1998;18:1946–1955. [pmid: 9528766]|
|122.||Yang X,Diehl AM,Wand GS. Ethanol exposure alters the phosphorylation of cyclic AMP responsive element binding protein and cyclic AMP responsive element binding activity in rat cerebellumJ. Pharmacol. Exp. Ther 1996;278:338–346. [pmid: 8764368]|
|123.||Yang X,Horn K,Baraban JM,Wand GS. Chronic ethanol administration decreases phosphorylation of cyclic AMP response element-binding protein in granule cells of rat cerebellumJ. Neurochem 1998;70:224–232. [pmid: 9422366]|
|124.||Yao WD,Gainetdinov RR,Arbuckle MI,Sotnikova TD,Cyr M,Beaulieu JM,Torres GE,Grant SG,Caron MG. Identification of PSD-95 as a regulator of dopamine-mediated synaptic and behavioral plasticityNeuron 2004;41:625–638. [pmid: 14980210]|
|125.||Zhou LF,Zhu YP. Changes of CREB in rat hippocampus, prefrontal cortex and nucleus accumbens during three phases of morphine induced conditioned place preference in ratsJ. Zhejiang. Univ. Sci. B 2006;7:107–113. [pmid: 16421965]|
Keywords: Key Words CREB, conditional knockout, addiction, cAMP response element binding protein, behavior..
Previous Document: Allosteric modulators of GABA(B) receptors: mechanism of action and therapeutic perspective.
Next Document: Effects of nicotine during pregnancy: human and experimental evidence.