Document Detail


Nuclear structure and chromosome segregation in Drosophila male meiosis depend on the ubiquitin ligase dTopors.
MedLine Citation:
PMID:  21900273     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In many organisms, homolog pairing and synapsis at meiotic prophase depend on interactions between chromosomes and the nuclear membrane. Male Drosophila lack synapsis, but nonetheless, their chromosomes closely associate with the nuclear periphery at prophase I. To explore the functional significance of this association, we characterize mutations in nuclear blebber (nbl), a gene required for both spermatocyte nuclear shape and meiotic chromosome transmission. We demonstrate that nbl corresponds to dtopors, the Drosophila homolog of the mammalian dual ubiquitin/small ubiquitin-related modifier (SUMO) ligase Topors. We show that mutations in dtopors cause abnormalities in lamin localizations, centriole separation, and prophase I chromatin condensation and also cause anaphase I bridges that likely result from unresolved homolog connections. Bridge formation does not require mod(mdg4) in meiosis, suggesting that bridges do not result from misregulation of the male homolog conjunction complex. At the ultrastructural level, we observe disruption of nuclear shape, an uneven perinuclear space, and excess membranous structures. We show that dTopors localizes to the nuclear lamina at prophase, and also transiently to intranuclear foci. As a role of dtopors at gypsy insulator has been reported, we also asked whether these new alleles affected expression of the gypsy-induced mutation ct(6) and found that it was unaltered in dtopors homozygotes. Our results indicate that dTopors is required for germline nuclear structure and meiotic chromosome segregation, but in contrast, is not necessary for gypsy insulator function. We suggest that dtopors plays a structural role in spermatocyte lamina that is critical for multiple aspects of meiotic chromosome transmission.
Authors:
Maiko Matsui; Krishn C Sharma; Carol Cooke; Barbara T Wakimoto; Mohammad Rasool; Miranda Hayworth; Christopher A Hylton; John E Tomkiel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-09-06
Journal Detail:
Title:  Genetics     Volume:  189     ISSN:  1943-2631     ISO Abbreviation:  Genetics     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-15     Completed Date:  2012-03-01     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  779-93     Citation Subset:  IM    
Affiliation:
Department of Biology, University of North Carolina, Greensboro, North Carolina 27402, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Anaphase / genetics
Animals
Cell Nucleus / enzymology,  genetics,  metabolism*
Centrioles / enzymology,  genetics,  metabolism
Chromatin / genetics,  metabolism
Chromosome Segregation* / genetics
Chromosomes, Insect / enzymology,  genetics*
DNA-Binding Proteins / genetics
Drosophila Proteins / chemistry,  genetics,  metabolism*
Drosophila melanogaster / cytology*,  enzymology*,  genetics,  metabolism
Epistasis, Genetic / genetics
Female
Humans
Male
Meiosis / genetics*
Mice
Molecular Sequence Data
Mutation
RNA-Binding Proteins / genetics
Ubiquitin-Protein Ligases / chemistry,  genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
GM079780-01A1/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Chromatin; 0/DNA-Binding Proteins; 0/Drosophila Proteins; 0/RNA-Binding Proteins; 0/modulo protein, Drosophila; EC 6.3.2.19/Topors protein, Drosophila; EC 6.3.2.19/Ubiquitin-Protein Ligases
Comments/Corrections

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