Document Detail

Nuclear repositioning precedes promoter accessibility and is linked to the switching frequency of a Plasmodium falciparum invasion gene.
MedLine Citation:
PMID:  23245319     Owner:  NLM     Status:  MEDLINE    
Variation of surface adhesins, such as the Plasmodium falciparum erythrocyte invasion ligand PfRh4, is critical for virulence and immune evasion in many microbes. While phenotypic switching is linked to transcriptional changes and chromatin function, the determinants of switching frequency remain poorly defined. By expressing a prokaryotic DNA methylase in P. falciparum, we directly assayed accessibility of transcriptionally active and silent chromatin at the PfRh4 locus. Parasites selected for in vivo PfRh4 activation show a reversible increase in promoter accessibility and exhibit perinuclear repositioning of the locus from a silent to a conserved activation domain. Forced activation of a proximal gene results in a similar repositioning of the PfRh4 locus, with a concomitant increase in PfRh4 activation in a subpopulation of parasites and promoter accessibility correlating with actively transcribed loci. Thus, nuclear repositioning is associated with increased P. falciparum switching frequency, while promoter accessibility is tightly linked to clonally active PfRh4 promoters.
Bradley I Coleman; Ulf Ribacke; Micah Manary; Amy K Bei; Elizabeth A Winzeler; Dyann F Wirth; Manoj T Duraisingh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell host & microbe     Volume:  12     ISSN:  1934-6069     ISO Abbreviation:  Cell Host Microbe     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-18     Completed Date:  2013-05-23     Revised Date:  2014-06-25    
Medline Journal Info:
Nlm Unique ID:  101302316     Medline TA:  Cell Host Microbe     Country:  United States    
Other Details:
Languages:  eng     Pagination:  739-50     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Antigenic Variation
Cell Nucleus / metabolism
Chromatin / metabolism
Membrane Proteins / genetics*
Plasmodium falciparum / genetics,  pathogenicity*
Promoter Regions, Genetic*
Protozoan Proteins / genetics*
Transcription, Genetic*
Virulence Factors / genetics*
Grant Support
Reg. No./Substance:
0/Chromatin; 0/Membrane Proteins; 0/Protozoan Proteins; 0/RH4 protein, Plasmodium falciparum; 0/Virulence Factors

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