Document Detail


Nuclear DNA fragmentation during cell death of short-lived ray tracheids in the conifer Pinus densiflora.
MedLine Citation:
PMID:  21052767     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
One key event in the programmed cell death is nuclear DNA fragmentation. We investigated the timing of nuclear DNA fragmentation during the cell death of short-lived ray tracheids in Pinus densiflora using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Fluorescence due to TUNEL was detected only in deformed nuclei that lacked obvious chromatin in ray tracheids that were adjacent to ray tracheids that no longer contained nuclei. Our observations revealed that nuclear DNA fragmentation occurred only at the final stage of cell death in ray tracheids in situ.
Authors:
Satoshi Nakaba; Takafumi Kubo; Ryo Funada
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Publication Detail:
Type:  Journal Article     Date:  2010-10-30
Journal Detail:
Title:  Journal of plant research     Volume:  124     ISSN:  1618-0860     ISO Abbreviation:  J. Plant Res.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9887853     Medline TA:  J Plant Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  379-84     Citation Subset:  IM    
Affiliation:
Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-Tokyo, 183-8509, Japan.
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