Document Detail

NtSCP1 from tobacco is an extracellular serine carboxypeptidase III that has an impact on cell elongation.
MedLine Citation:
PMID:  22214816     Owner:  NLM     Status:  MEDLINE    
The leaf extracellular space contains several peptidases, most of which are of unknown function. We isolated cDNAs for two extracellular serine carboxypeptidase III genes from tobacco (Nicotiana tabacum), NtSCP1 and NtSCP2, belonging to a phylogenetic clade not yet functionally characterized in plants. NtSCP1 and NtSCP2 are orthologs derived from the two ancestors of tobacco. Reverse transcription-polymerase chain reaction analysis showed that NtSCP1 and NtSCP2 are expressed in root, stem, leaf, and flower tissues. Expression analysis of the β-glucuronidase reporter gene fused to the NtSCP1 transcription promoter region confirmed this expression profile. Western blotting of NtSCP1 and expression of an NtSCP1-green fluorescent protein fusion protein showed that the protein is located in the extracellular space of tobacco leaves and culture cells. Purified His-tagged NtSCP1 had carboxypeptidase activity in vitro. Transgenic tobacco plants overexpressing NtSCP1 showed a reduced flower length due to a decrease in cell size. Etiolated seedlings of these transgenic plants had shorter hypocotyls. These data provide support for a role of an extracellular type III carboxypeptidase in the control of cell elongation.
Manuela Désirée Bienert; Mélanie Delannoy; Catherine Navarre; Marc Boutry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-03
Journal Detail:
Title:  Plant physiology     Volume:  158     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-07     Completed Date:  2012-06-21     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1220-9     Citation Subset:  IM    
Institut des Sciences de la Vie, University of Louvain, 1348 Louvain-la-Neuve, Belgium.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/ABQ65859;  ABQ65860;  GU734644
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MeSH Terms
Amino Acid Sequence
Carboxypeptidases / genetics,  metabolism*
Cell Enlargement*
Cloning, Molecular
Culture Media / metabolism
DNA, Complementary / genetics,  metabolism
DNA, Plant / genetics,  metabolism*
Enzyme Activation
Escherichia coli / genetics,  metabolism
Extracellular Space / genetics,  metabolism
Gene Expression Regulation, Plant
Genes, Reporter
Green Fluorescent Proteins / metabolism
Molecular Sequence Data
Plant Components, Aerial / genetics,  growth & development,  metabolism
Plant Proteins / genetics,  metabolism*
Plant Roots / genetics,  growth & development,  metabolism
Plants, Genetically Modified / enzymology,  genetics,  growth & development
Recombinant Fusion Proteins / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tobacco / enzymology*,  genetics,  growth & development
Reg. No./Substance:
0/Culture Media; 0/DNA, Complementary; 0/DNA, Plant; 0/Plant Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; EC 3.4.-/Carboxypeptidases; EC carboxypeptidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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