Document Detail

Nrf2-mediated protection against UVA radiation in human skin keratinocytes.
MedLine Citation:
PMID:  21422597     Owner:  NLM     Status:  MEDLINE    
Ultraviolet A (UVA, 320-400 nm) radiation is an oxidizing agent that causes significant damage to cellular components and that leads to photoaging and cancer. It strongly induces NF-E2-related factor 2 (Nrf2) expressions in cultured FEK4 human skin fibroblasts but weakly induces it in transformed HaCaT skin keratinocytes. Nrf2 silencing increases cell damage at a moderate dose of UVA irradiation (250 kJ•m(-2)) in FEK4 fibroblasts, but whether a decrease in Nrf2 sensitizes HaCaT keratinocytes to a moderate to high dose (250-500 kJ•m(-2)) of UVA irradiation (i.e., 400 kJ•m(-2), peak emission 365 nm) is currently unknown. A moderate to high dose of UVA irradiation only slightly increased Nrf2 expression in HaCaT skin keratinocytes. Knockdown of Nrf2 by specific silencing of Nrf2 (siNrf2) strongly increased cell damage as gauged by membrane damage (LDH) and cell viability (MTT assay) following this dose of UVA irradiation. These results suggest that decreased Nrf2 significantly increased UVA irradiation-induced cell damage in skin keratinocytes. Nrf2 may play a role in protecting human skin keratinocytes from UVA radiation-induced damage.
F F Tian; F F Zhang; X D Lai; L J Wang; L Yang; X Wang; G Singh; J L Zhong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience trends     Volume:  5     ISSN:  1881-7823     ISO Abbreviation:  Biosci Trends     Publication Date:  2011  
Date Detail:
Created Date:  2011-03-22     Completed Date:  2011-07-15     Revised Date:  2011-12-28    
Medline Journal Info:
Nlm Unique ID:  101502754     Medline TA:  Biosci Trends     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  23-9     Citation Subset:  IM    
The "111 Project" Laboratory of Biomechanics & Tissue Repair Engineering, Ministry of Education, College of Bioengineering, Chongqing University, China.
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MeSH Terms
Blotting, Western
Dose-Response Relationship, Radiation
Fluorescent Antibody Technique
Gene Knockdown Techniques
Keratinocytes / metabolism*,  pathology,  radiation effects*
NF-E2-Related Factor 2 / genetics,  metabolism*
RNA Interference
Ultraviolet Rays / adverse effects*
Reg. No./Substance:
0/NF-E2-Related Factor 2; 0/NFE2L2 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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