| Nrf2-dependent and -independent responses to nitro-fatty acids in human endothelial cells: identification of heat shock response as the major pathway activated by nitro-oleic acid. | |
| | |
MedLine Citation:
|
PMID: 19808663 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Electrophilic fatty acid derivatives, including nitrolinoleic acid and nitro-oleic acid (OA-NO(2)), can mediate anti-inflammatory and pro-survival signaling reactions. The transcription factor Nrf2, activated by electrophilic fatty acids, suppresses redox-sensitive pro-inflammatory gene expression and protects against vascular endothelial oxidative injury. It was therefore postulated that activation of Nrf2 by OA-NO(2) accounts in part for its anti-inflammatory actions, motivating the characterization of Nrf2-dependent and -independent effects of OA-NO(2) on gene expression using genome-wide transcriptional profiling. Control and Nrf2-small interfering RNA-transfected human endothelial cells were treated with vehicle, oleic acid, or OA-NO(2), and differential gene expression profiles were determined. Although OA-NO(2) significantly induced the expression of Nrf2-dependent genes, including heme oxygenase-1 and glutamate-cysteine ligase modifier subunit, the majority of OA-NO(2)-regulated genes were regulated by Nrf2-independent pathways. Moreover, gene set enrichment analysis revealed that the heat shock response is the major pathway activated by OA-NO(2), with robust induction of a number of heat shock genes regulated by the heat shock transcription factor. Inasmuch as the heat shock response mediates anti-inflammatory and cytoprotective actions, this mechanism is proposed to contribute to the protective cell signaling functions of nitro-fatty acids and other electrophilic fatty acid derivatives. |
| | |
Authors:
|
Emilia Kansanen; Henna-Kaisa Jyrkkänen; Oscar L Volger; Hanna Leinonen; Annukka M Kivelä; Sanna-Kaisa Häkkinen; Steven R Woodcock; Francisco J Schopfer; Anton J Horrevoets; Seppo Ylä-Herttuala; Bruce A Freeman; Anna-Liisa Levonen |
Related Documents
:
|
7623043 - Studies on the inhibitory effects of caffeoylquinic acids on monocyte migration and sup... 12912963 - Tannic acid is an inhibitor of cxcl12 (sdf-1alpha)/cxcr4 with antiangiogenic activity. 3227823 - Antagonistic action of reductants against vanadate-induced ep decrease. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-10-05 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 284 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-11-25 Completed Date: 2010-01-21 Revised Date: 2012-05-28 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 33233-41 Citation Subset: IM |
Affiliation:
|
Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, FIN-70211 Kuopio, Finland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blotting, Western Cell Line Cells, Cultured Cluster Analysis Dose-Response Relationship, Drug Endothelial Cells / cytology, drug effects*, metabolism Gene Expression / drug effects Gene Expression Profiling Glutamate-Cysteine Ligase / genetics, metabolism Heme Oxygenase-1 / genetics, metabolism Hot Temperature Humans Linoleic Acids / pharmacology* NAD(P)H Dehydrogenase (Quinone) / genetics, metabolism NF-E2-Related Factor 2 / genetics*, metabolism Nitro Compounds / pharmacology* Oleic Acids / pharmacology* Oligonucleotide Array Sequence Analysis RNA Interference Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / drug effects |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HL58115/HL/NHLBI NIH HHS; R01 HL64937/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/10-nitro-9,12-octadecadienoic acid; 0/Linoleic Acids; 0/NF-E2-Related Factor 2; 0/NFE2L2 protein, human; 0/Nitro Compounds; 0/Oleic Acids; EC 1.14.99.3/HMOX1 protein, human; EC 1.14.99.3/Heme Oxygenase-1; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone); EC 1.6.5.2/NQO1 protein, human; EC 6.3.2.2/Glutamate-Cysteine Ligase; EC 6.3.2.2/glutamate-cysteine ligase modifier subunit, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evidence that nucleosomes inhibit mismatch repair in eukaryotic cells.
Next Document: A double tyrosine motif in the cardiac sodium channel domain III-IV linker couples calcium-dependent...