| Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity. | |
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MedLine Citation:
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PMID: 6219989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Novikoff ascites tumor cell homogenate was found to catalyze the transfer of [14C]N-acetylglucosamine from UDP-[14C]GlcNAc to asialo-alpha 1-acid glycoprotein. Mucins appeared to be poor acceptors. Methylation and hydrolysis of the product formed in an incubation with UDP-GlcNAc and asialo-alpha 1-acid [3H]glycoprotein yielded 2,4,6-trimethyl [3H]galactose and 2,3,4-trimethyl [3H]galactose, indicating that N-acetylglucosaminyl residues were introduced to position C-3 and C-6 of the terminal galactoses on the glycoprotein. It is concluded that Novikoff cells contain two N-acetylglucosaminyltransferases which might be involved in the synthesis of linear and branched forms of cell surface polylactosaminoglycans and blood group I/i antigenic structures. |
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Authors:
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D H van den Eijnden; H Winterwerp; P Smeeman; W E Schiphorst |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 258 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1983 Mar |
Date Detail:
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Created Date: 1983-05-05 Completed Date: 1983-05-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3435-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cations, Divalent Kinetics Liver Neoplasms, Experimental / enzymology* N-Acetylglucosaminyltransferases / metabolism* Rats Rats, Inbred Strains Substrate Specificity |
| Grant Support | |
ID/Acronym/Agency:
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HE 13881//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Cations, Divalent; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC 2.4.1.149/N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; EC 2.4.1.150/N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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