Document Detail


Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity.
MedLine Citation:
PMID:  6219989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Novikoff ascites tumor cell homogenate was found to catalyze the transfer of [14C]N-acetylglucosamine from UDP-[14C]GlcNAc to asialo-alpha 1-acid glycoprotein. Mucins appeared to be poor acceptors. Methylation and hydrolysis of the product formed in an incubation with UDP-GlcNAc and asialo-alpha 1-acid [3H]glycoprotein yielded 2,4,6-trimethyl [3H]galactose and 2,3,4-trimethyl [3H]galactose, indicating that N-acetylglucosaminyl residues were introduced to position C-3 and C-6 of the terminal galactoses on the glycoprotein. It is concluded that Novikoff cells contain two N-acetylglucosaminyltransferases which might be involved in the synthesis of linear and branched forms of cell surface polylactosaminoglycans and blood group I/i antigenic structures.
Authors:
D H van den Eijnden; H Winterwerp; P Smeeman; W E Schiphorst
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  258     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3435-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cations, Divalent
Kinetics
Liver Neoplasms, Experimental / enzymology*
N-Acetylglucosaminyltransferases / metabolism*
Rats
Rats, Inbred Strains
Substrate Specificity
Grant Support
ID/Acronym/Agency:
HE 13881//PHS HHS
Chemical
Reg. No./Substance:
0/Cations, Divalent; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC 2.4.1.149/N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; EC 2.4.1.150/N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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