| Novel use of biodegradable casein conduits for guided peripheral nerve regeneration. | |
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MedLine Citation:
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PMID: 21525148 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent advances in nerve repair technology have focused on finding more biocompatible, non-toxic materials to imitate natural peripheral nerve components. In this study, casein protein cross-linked with naturally occurring genipin (genipin-cross-linked casein (GCC)) was used for the first time to make a biodegradable conduit for peripheral nerve repair. The GCC conduit was dark blue in appearance with a concentric and round lumen. Water uptake, contact angle and mechanical tests indicated that the conduit had a high stability in water and did not collapse and cramped with a sufficiently high level of mechanical properties. Cytotoxic testing and terminal deoxynucleotidyl transferase dUTP nick-end labelling assay showed that the GCC was non-toxic and non-apoptotic, which could maintain the survival and outgrowth of Schwann cells. Non-invasive real-time nuclear factor-κB bioluminescence imaging accompanied by histochemical assessment showed that the GCC was highly biocompatible after subcutaneous implantation in transgenic mice. Effectiveness of the GCC conduit as a guidance channel was examined as it was used to repair a 10 mm gap in the rat sciatic nerve. Electrophysiology, labelling of calcitonin gene-related peptide in the lumbar spinal cord, and histology analysis all showed a rapid morphological and functional recovery for the disrupted nerves. Therefore, we conclude that the GCC can offer great nerve regeneration characteristics and can be a promising material for the successful repair of peripheral nerve defects. |
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Authors:
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Shih-Wei Hsiang; Chin-Chuan Tsai; Fuu-Jen Tsai; Tin-Yun Ho; Chun-Hsu Yao; Yueh-Sheng Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-04-27 |
Journal Detail:
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Title: Journal of the Royal Society, Interface / the Royal Society Volume: 8 ISSN: 1742-5662 ISO Abbreviation: J R Soc Interface Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-09-28 Completed Date: 2012-01-13 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 101217269 Medline TA: J R Soc Interface Country: England |
Other Details:
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Languages: eng Pagination: 1622-34 Citation Subset: IM |
Affiliation:
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Laboratory of Biomaterials, School of Chinese Medicine, China Medical University, Taichung, Taiwan, Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorbable Implants* Animals Caseins / therapeutic use* Electrophysiology Guided Tissue Regeneration / instrumentation*, methods Histological Techniques Image Processing, Computer-Assisted Immunohistochemistry In Situ Nick-End Labeling Iridoid Glycosides / therapeutic use Iridoids Mice Mice, Transgenic Nerve Regeneration / physiology* Peripheral Nerve Injuries / therapy* Rats Schwann Cells / cytology, physiology* Sciatic Nerve / injuries* |
| Chemical | |
Reg. No./Substance:
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0/Caseins; 0/Iridoid Glycosides; 0/Iridoids; 6902-77-8/genipin |
| Comments/Corrections | |
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