Document Detail

Novel use of biodegradable casein conduits for guided peripheral nerve regeneration.
MedLine Citation:
PMID:  21525148     Owner:  NLM     Status:  MEDLINE    
Recent advances in nerve repair technology have focused on finding more biocompatible, non-toxic materials to imitate natural peripheral nerve components. In this study, casein protein cross-linked with naturally occurring genipin (genipin-cross-linked casein (GCC)) was used for the first time to make a biodegradable conduit for peripheral nerve repair. The GCC conduit was dark blue in appearance with a concentric and round lumen. Water uptake, contact angle and mechanical tests indicated that the conduit had a high stability in water and did not collapse and cramped with a sufficiently high level of mechanical properties. Cytotoxic testing and terminal deoxynucleotidyl transferase dUTP nick-end labelling assay showed that the GCC was non-toxic and non-apoptotic, which could maintain the survival and outgrowth of Schwann cells. Non-invasive real-time nuclear factor-κB bioluminescence imaging accompanied by histochemical assessment showed that the GCC was highly biocompatible after subcutaneous implantation in transgenic mice. Effectiveness of the GCC conduit as a guidance channel was examined as it was used to repair a 10 mm gap in the rat sciatic nerve. Electrophysiology, labelling of calcitonin gene-related peptide in the lumbar spinal cord, and histology analysis all showed a rapid morphological and functional recovery for the disrupted nerves. Therefore, we conclude that the GCC can offer great nerve regeneration characteristics and can be a promising material for the successful repair of peripheral nerve defects.
Shih-Wei Hsiang; Chin-Chuan Tsai; Fuu-Jen Tsai; Tin-Yun Ho; Chun-Hsu Yao; Yueh-Sheng Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-27
Journal Detail:
Title:  Journal of the Royal Society, Interface / the Royal Society     Volume:  8     ISSN:  1742-5662     ISO Abbreviation:  J R Soc Interface     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-09-28     Completed Date:  2012-01-13     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101217269     Medline TA:  J R Soc Interface     Country:  England    
Other Details:
Languages:  eng     Pagination:  1622-34     Citation Subset:  IM    
Laboratory of Biomaterials, School of Chinese Medicine, China Medical University, Taichung, Taiwan, Republic of China.
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MeSH Terms
Absorbable Implants*
Caseins / therapeutic use*
Guided Tissue Regeneration / instrumentation*,  methods
Histological Techniques
Image Processing, Computer-Assisted
In Situ Nick-End Labeling
Iridoid Glycosides / therapeutic use
Mice, Transgenic
Nerve Regeneration / physiology*
Peripheral Nerve Injuries / therapy*
Schwann Cells / cytology,  physiology*
Sciatic Nerve / injuries*
Reg. No./Substance:
0/Caseins; 0/Iridoid Glycosides; 0/Iridoids; 6902-77-8/genipin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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