Document Detail


Novel two-stage screening procedure leads to the identification of a new class of transfection enhancers.
MedLine Citation:
PMID:  16532511     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Non-viral gene transfer efficiency is low as compared to viral vector systems. Here we describe the discovery of new drugs that are capable of enhancing non-viral gene transfer into mammalian cells using a novel two-stage screening procedure. METHODS: First, potential candidates are preselected from a molecular library at various concentrations by a semi-automated yeast transfection screen (YTS). The maximal transfection efficiency of every positive drug is subsequently determined in independent experiments at the optimal concentration and compared to the inhibitory effect of the drug on cell growth (IC50). In a subsequent mammalian cell transfection screen (MTS), the maximal transfection efficiency and the IC50 are determined for all preselected drugs using a human cell line and a luciferase reporter gene construct. RESULTS: Employing our novel system we have been able to identify a new class of transfection enhancers, the tricyclic antidepressants (i.e. doxepin, maprotiline, desipramine and amoxapine). All positive drugs enhanced gene transfer in both yeast and human cell lines, but lower concentrations were sufficient for mammalian cells. With a triple combination of doxepin, amoxapine and chloroquine we obtained a transfection efficiency that exceeded that of chloroquine, one of the best-known transfection enhancers of mammalian cells, by nearly one order of magnitude. CONCLUSIONS: Non-viral gene transfer efficiency can be increased significantly using new transfection enhancers that are identified by a novel, semi-automated two-stage screening system employing yeast cells in the first and specific human target cells in the second round.
Authors:
Birgit Neukamm; Andreas Weimann; Shuling Wu; Margrete Danevad; Christine Lang; Reinhard Gessner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of gene medicine     Volume:  8     ISSN:  1099-498X     ISO Abbreviation:  J Gene Med     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-29     Completed Date:  2006-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815764     Medline TA:  J Gene Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  745-53     Citation Subset:  IM    
Copyright Information:
Copyright 2006 John Wiley & Sons, Ltd.
Affiliation:
Institute of Microbiology and Genetics, Technical University Berlin, Gustav-Meyer-Allee 25, D-13355 Berlin.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical / methods*
Humans
Mammals / genetics
Saccharomyces cerevisiae / drug effects
Transfection / methods*

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