| Novel surface modified molecularly imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers for selective recognition of lysozyme in aqueous solution. | |
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MedLine Citation:
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PMID: 19361806 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel protein imprinted polymer for selective recognition of lysozyme was obtained. Acryloyl-beta-cyclodextrin, which offered hydrophilic exterior and hydrophobic cavity that were allowed to self-assemble with the template protein through hydrogen interaction and hydrophobic interaction, was synthesized and used as the functional monomer. Polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a new type of protein imprinted polymer. Langmuir adsorption model was employed to describe the isotherms, and maximum adsorption capacity was evaluated. The performance of such imprinted polymer was further demonstrated by high-performance liquid chromatography, and the results showed that the column packed with the lysozyme imprinted silica beads could effectively separate lysozyme from the mixture of lysozyme-cytochrome c, lysozyme-bovine serum albumin, lysozyme-avidin or lysozyme-methylated bovine serum albumin, which showed its high selectivity. |
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Authors:
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Wei Zhang; Lei Qin; Xi-Wen He; Wen-You Li; Yu-Kui Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-25 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1216 ISSN: 1873-3778 ISO Abbreviation: J Chromatogr A Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-04 Completed Date: 2009-06-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 4560-7 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Nankai University, Tianjin, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrylamide
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chemistry* Acrylic Resins / chemistry* Adsorption Animals Cattle Chromatography, High Pressure Liquid Elements Microscopy, Electron, Scanning Microspheres Molecular Imprinting / methods* Muramidase / analysis* Nitrogen / chemistry Polymers / chemistry* Serum Albumin, Bovine / chemistry Silicon Dioxide / chemistry Solutions Surface Properties Temperature beta-Cyclodextrins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Elements; 0/Polymers; 0/Serum Albumin, Bovine; 0/Solutions; 0/beta-Cyclodextrins; 7585-39-9/betadex; 7631-86-9/Silicon Dioxide; 7727-37-9/Nitrogen; 79-06-1/Acrylamide; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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