Document Detail


Novel solid protein solder designs for laser-assisted tissue repair.
MedLine Citation:
PMID:  10960821     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: Previous studies have shown that the application of chromophore-enhanced albumin protein solders to augment laser tissue repairs significantly improves repair strength, enhances edge co-optation, and reduces thermal tissue injury. These investigations are furthered with this in vitro study conducted to assess a new range of specially designed chromophore-enhanced solid protein solders manufactured and tested for application during laser-assisted tissue repair. STUDY DESIGN/MATERIALS AND METHODS: The experimental study was divided into three parts. In the first part of the study, the creation of a chromophore concentration gradient across the thickness of the solid protein solder was investigated as a means to improve control of the heat source gradient through the solder during laser irradiation. In the second part of the study, predenaturation of the solid protein solder was investigated as a means for enhancing the stability of the solder in physiological fluids before irradiation. Finally, in the third part of the study, the feasibility of using synthetic polymers as a scaffold for traditional albumin protein solder mixes was investigated as a means of improving the flexibility of the solder. RESULTS: Uniform denaturation across the thickness of the solder was achieved by controlling the chromophore concentration gradient, thus ensuring stable solder-tissue fusion when the specimen was submerged in a hydrated environment. Predenaturation of the solid protein solder significantly reduced the solubility of the solder, and consequently, improved the handling characteristics of the solder. The solder-doped polymer membranes were flexible enough to be wrapped around tissue, whereas their solid nature avoided problems associated with "runaway" of the less viscous liquid solders currently used by researchers. In addition, the solder-doped polymer membranes could be easily tailored to a wide range of geometries suitable to many clinical applications. CONCLUSION: The novel solid protein solder designs presented here add a new dimension to tissue repair as their flexible, moldable, and absorption controllable nature, greatly improves the clinical applicability of laser-assisted tissue repair.
Authors:
K M McNally; B S Sorg; A J Welch
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Lasers in surgery and medicine     Volume:  27     ISSN:  0196-8092     ISO Abbreviation:  Lasers Surg Med     Publication Date:  2000  
Date Detail:
Created Date:  2000-10-11     Completed Date:  2000-10-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8007168     Medline TA:  Lasers Surg Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  147-57     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Wiley-Liss, Inc.
Affiliation:
Biomedical Engineering Laser Laboratory, The University of Texas at Austin, Austin, Texas, USA. karen.mcnally@rose-hulman.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta, Thoracic / pathology,  surgery*
Biocompatible Materials / therapeutic use
Cattle
Coloring Agents / therapeutic use
Feasibility Studies
Indocyanine Green / therapeutic use
Lactic Acid / therapeutic use
Laser Therapy / methods*
Microscopy, Electron, Scanning
Polyglycolic Acid / therapeutic use
Polymers / therapeutic use
Serum Albumin, Bovine / chemistry*,  therapeutic use*
Solubility
Temperature
Tensile Strength
Tissue Adhesives / chemistry*
Vascular Surgical Procedures / methods*
Wound Healing*
Wounds and Injuries / surgery
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Coloring Agents; 0/Polymers; 0/Serum Albumin, Bovine; 0/Tissue Adhesives; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 3599-32-4/Indocyanine Green; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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