Document Detail


Novel sialic acid transporter of Haemophilus influenzae.
MedLine Citation:
PMID:  16113244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nontypeable Haemophilus influenzae is an opportunistic pathogen and a common cause of otitis media in children and of chronic bronchitis and pneumonia in patients with chronic obstructive pulmonary disease. The lipooligosaccharides, a major component of the outer membrane of H. influenzae, play an important role in microbial virulence and pathogenicity. N-Acetylneuraminic acid (sialic acid) can be incorporated into the lipooligosaccharides as a terminal nonreducing sugar. Although much of the pathway of sialic acid incorporation into lipooligosaccharides is understood, the transporter responsible for N-acetylneuraminic acid uptake in H. influenzae has yet to be characterized. In this paper we demonstrate that this transporter is a novel sugar transporter of the tripartite ATP-independent periplasmic transporter family. In the absence of this transporter, H. influenzae cannot incorporate sialic acid into its lipooligosaccharides, making the organism unable to survive when exposed to human serum and causing reduced viability in biofilm growth.
Authors:
Simon Allen; Anthony Zaleski; Jason W Johnston; Bradford W Gibson; Michael A Apicella
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  73     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-22     Completed Date:  2005-10-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5291-300     Citation Subset:  IM    
Affiliation:
Buck Institute for Age Research, 8001 Redwood Blvd., Novato, California 94945, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ054469;  DQ054470;  DQ054471
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MeSH Terms
Descriptor/Qualifier:
Biofilms
Biological Transport
Electrophoresis, Polyacrylamide Gel
Haemophilus influenzae / genetics,  metabolism*
Lipopolysaccharides / metabolism
Molecular Sequence Data
N-Acetylneuraminic Acid / metabolism*
Organic Anion Transporters / genetics,  isolation & purification,  metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Symporters / genetics,  isolation & purification,  metabolism*
Grant Support
ID/Acronym/Agency:
AI24616/AI/NIAID NIH HHS; AI30040/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 0/Organic Anion Transporters; 0/Symporters; 0/lipid-linked oligosaccharides; 0/sialic acid transport proteins; 131-48-6/N-Acetylneuraminic Acid
Comments/Corrections

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